Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters
Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters
复制标题
DOI：
10.1111/1574-6941.12135
复制
发表时间：
2013-09-01
影响因子：
4.2
通讯作者：
Gomes, Newton C. M.
中科院分区：
文献类型：
--
作者：
Cleary, Daniel F. R.;Becking, Leontine E.;Gomes, Newton C. M.
海洋湖泊是独特的生态系统，其中包含孤立的海洋生物种群。许多湖泊与周围的海洋栖息地隔绝，栖息着许多特有物种。在这项研究中，首次使用 16S rRNA 基因扩增子的条形码焦磷酸测序技术对居住在这些湖泊中的海绵微生物群落进行了研究。我们的主要目标是比较栖息在海洋湖泊和邻近开放沿海系统的两种海绵物种（Suberites diversicolor 和 Cinachyrella australiensis）的细菌丰富度和组成。宿主物种和栖息地解释了近 59% 的细菌组成变化。两种宿主物种之间的组成存在显着差异。在杂色链球菌中，湖泊内外的细菌群落几乎没有明显的差异。此外，该物种的细菌群落以三种非常密切相关的α变形菌类群为主（占所有序列的63%），这些类群被鉴定为属于最近描述的千菌目。相比之下，澳大利亚 Cinachyrella 则在湖泊内外拥有明显不同的细菌群落，几乎没有共享丰富的类群。开放生境中的 Cinachyrella australiensis 与湖泊生境中的 Cinachyrella australiensis 仅共享 9.4% 的 OTU。因此，细菌具有高度的物种特异性，就澳大利亚隐球菌而言，还具有栖息地特异性。
Marine lakes are unique ecosystems that contain isolated populations of marine organisms. Isolated from the surrounding marine habitat, many lakes house numerous endemic species. In this study, microbial communities of sponges inhabiting these lakes were investigated for the first time using barcoded pyrosequencing of 16S rRNA gene amplicons. Our main goals were to compare the bacterial richness and composition of two sponge species (Suberites diversicolor and Cinachyrella australiensis) inhabiting both marine lakes and adjacent open coastal systems. Host species and habitat explained almost 59% of the variation in bacterial composition. There was a significant difference in composition between both host species. Within S. diversicolor, there was little discernible difference between bacterial communities inside and outside lakes. The bacterial community of this species was, furthermore, dominated (63% of all sequences) by three very closely related alphaproteobacterial taxa identified as belonging to the recently described order Kiloniellales. Cinachyrella australiensis, in contrast, hosted markedly different bacterial communities inside and outside lakes with very few shared abundant taxa. Cinachyrella australiensis in open habitat only shared 9.4% of OTUs with C. australiensis in lake habitat. Bacteria were thus both highly species specific and, in the case of C. australiensis, habitat specific.