Spiral Layer-by-Layer Micro-Nanostructured Scaffolds for Bone Tissue Engineering
Spiral Layer-by-Layer Micro-Nanostructured Scaffolds for Bone Tissue Engineering
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DOI：
10.1021/acsbiomaterials.8b00393
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发表时间：
2018-06-01
影响因子：
5.8
通讯作者：
Kumbar, Sangamesh G.
中科院分区：
文献类型：
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作者：
Manoukian, Ohan S.;Aravamudhan, Aja;Kumbar, Sangamesh G.
本文报道了纳米纤维和羟基磷灰石（HA）复合微纳米螺旋结构骨再生支架的制备和表征。螺旋状聚（乳酸-共-乙醇酸）（PLGA）多孔微结构被稀疏间隔的PLGA纳米纤维和HA涂覆以增加表面积和生物活性。以逐层（LBL）方式的基于聚电解质的HA涂层允许10-70 μ M Ca 2 +/mm（2）掺入。这些支架提供了一个控制释放的钙离子长达60天，不同的释放动力学占高达10-50 μ g。与对照微结构相比，螺旋支架支持大鼠骨髓基质细胞（MSCs）的上级粘附、增殖和成骨分化。螺旋形微纳米结构支持均匀的组织向内生长，并导致早在3周的兔尺骨骨缺损模型中支架中心的骨岛形成。相比之下，对照圆柱形支架显示组织向内生长仅在表面，因为支架运输功能的限制。
This Article reports the fabrication and characterization of composite micro-nanostructured spiral scaffolds functionalized with nanofibers and hydroxyapatite (HA) for bone regeneration. The spiral poly(lactic acid-co-glycolic acid) (PLGA) porous microstructure was coated with sparsely spaced PLGA nanofibers and HA to enhance surface area and bioactivity. Polyelectrolyte-based HA coating in a layer-by-layer (LBL) fashion allowed 10-70 mu M Ca2+/mm(2) incorporation. These scaffolds provided a controlled release of Ca2+ ions up to 60 days with varied release kinetics accounting up to 10-50 mu g. Spiral scaffolds supported superior adhesion, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (MSCs) as compared to controls microstructures. Spiral micro-nanostructures supported homogeneous tissue ingrowth and resulted in bone-island formation in the center of the scaffold as early as 3 weeks in a rabbit ulnar bone defect model. In contrast, control cylindrical scaffolds showed tissue ingrowth only at the surface because of limitations in scaffold transport features.