Tropane alkaloid production in cultured cells of Duboisia leichhardtii
Tropane alkaloid production in cultured cells of Duboisia leichhardtii
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Duboisia leichhardtii 培养细胞中托烷生物碱的生产
DOI：
10.1007/bf00270196
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发表时间：
1984
影响因子：
6.2
通讯作者：
T. Endo
中科院分区：
文献类型：
--
作者：
Yasuyuki Yamada;T. Endo
愈伤组织来源于一种新的植物--杜柏实（Duboisia leichhardtii F.）穆勒厂经过3个月的文化，我们检测到少量的莨菪碱和东莨菪碱在无组织的愈伤组织，但数量下降，直到他们无法检测到连续的继代培养。当芽产生的无组织的愈伤组织，东莨菪碱加入到媒体中的转换能力出现;当根开始从拍摄组织愈伤组织，莨菪碱，东莨菪碱，尼古丁合成。培养不定根产生这些生物碱和转换外源莨菪碱东莨菪碱。
Calluses were derived from explants of aerial parts of a Duboisia leichhardtii F. Muell. plant. After 3 months of culture we detected small amounts of hyoscyamine and scopolamine in the unorganized calluses, but the amounts decreased until they were undetectable with successive subculture. When shoots were produced from the unorganized calluses, the ability to convert hyoscyamine added into media to scopolamine appeared; when roots were initiated from the shoot-organizing calluses, hyoscyamine, scoporamine, and nicotine were synthesized. Cultured adventitious roots produced these alkaloids and converted exogenous hyoscyamine to scopolamine.