Biological responses of human osteoblasts and osteoclasts to flame-sprayed coatings of hydroxyapatite and fluorapatite blends
Biological responses of human osteoblasts and osteoclasts to flame-sprayed coatings of hydroxyapatite and fluorapatite blends
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DOI：
10.1016/j.actbio.2009.10.029
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发表时间：
2010-04-01
影响因子：
9.7
通讯作者：
Haynes, D. R.
中科院分区：
文献类型：
--
作者：
Bhadang, K. A.;Holding, C. A.;Haynes, D. R.
本研究的目的是确定人成骨细胞和破骨细胞对热喷涂羟基磷灰石和氟磷灰石机械混合物基质的反应，以期确定骨整合的最佳混合物比例。人成骨细胞和破骨细胞在钛合金圆盘上生长，表面涂覆羟基磷灰石和氟磷灰石混合物，浓度范围为0~100%氟磷灰石。人成骨细胞在含40%氟磷灰石的混合物上附着更多，增殖速度更快。在含40%氟磷灰石的混合物中培养7天的人成骨细胞也表达了与骨代谢调节(骨保护素和受体激活剂核因子kappa B配体)和骨形成(骨桥蛋白、骨联素和骨唾液酸蛋白1)有关的几种蛋白质的最高水平的mRNA。破骨细胞吸收牙本质，但吸收羟基磷灰石-氟磷灰石混合物很差，特别是在高水平的氟磷灰石。这项体外研究表明，热喷涂含40%氟磷灰石的羟基磷灰石涂层可以促进最佳的骨生长和改善种植体的骨整合。(C)2009年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The aim of this study was to determine how the activities of human osteoblastic cells and osteoclasts respond to substrates of thermal-sprayed mechanical blends of hydroxyapatite and fluorapatite with a view of determining an optimal blend ratio for osseointegration. Human osteoblastic cells and osteoclasts were grown on titanium alloy discs coated with blends of hydroxyapatite and fluorapatite, with concentrations ranging from 0 to 100% fluorapatite. Human osteoblastic cells attached in greater numbers and proliferated at a greater rate on blends containing 40% fluorapatite. Human osteoblastic cells grown on blends containing 40% fluorapatite for 7 days also expressed the highest levels of mRNA for several proteins involved with regulating bone metabolism (osteoprotegerin and receptor activator nuclear factor kappa B ligand), and bone formation (osteopontin, osteonectin and bone sialoprotein 1). Osteoclasts resorbed the dentine but poorly resorbed the hydroxyapatite-fluorapatite blends, particularly at high levels of fluorapatite. This in vitro study demonstrates that thermal-sprayed hydroxyapatite coatings containing 40% fluorapatite may promote optimal bone growth and improve osseointegration of implants. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.