Inductively coupled, mm-sized, single channel optical neuro-stimulator with intensity enhancer
Inductively coupled, mm-sized, single channel optical neuro-stimulator with intensity enhancer
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DOI：
10.1038/s41378-019-0061-6
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发表时间：
2019-06
影响因子：
7.9
通讯作者：
W. Khan;Y. Jia;F. Madi;A. Weber;Maysam Ghovanloo;Wen Li
中科院分区：
文献类型：
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作者：
W. Khan;Y. Jia;F. Madi;A. Weber;Maysam Ghovanloo;Wen Li
我们介绍了一种单通道神经刺激器，由一个反射器耦合的微型发光二极管（µLED）和一个集成的毫米级无线接收器（Rx）线圈组成，用于自由浮动、无电池、无束缚的光遗传学神经调节。该系统采用双线圈感应链路，以低工作频率（<100 MHz）输送瞬时功率，以实现连续光学刺激，最大限度地减少侵入性和组织暴露于电磁辐射。与裸µLED相比，将微尺度反射器耦合到µLED可显著增强光强度。我们的激活刺激器的工作温度升高<1 °C，远低于生物医学植入物的安全限值。在体实验和组织学分析验证了无线光刺激在大鼠初级视皮层的有效性，使用c-Fos生物标志物作为光诱发神经元活动的报告者。
We introduce a single channel neuro-stimulator consisting of a reflector-coupled microscale light emitting diode (µLED) with an integrated mm-sized wireless receiver (Rx) coil for free-floating, battery-free, untethered optogenetics neuromodulation. The system utilizes a two-coil inductive link to deliver instantaneous power at a low operating frequency (<100 MHz) for continuous optical stimulation with minimized invasiveness and tissue exposure to electromagnetic radiation. Coupling a microscale reflector to the µLED provides significant light intensity enhancement compared to a bare µLED. Our activated stimulators have an operational temperature increase of <1 °C, well below the safety limit of biomedical implants. In vivo experiment and histological analysis verify the efficacy of wireless optical stimulation in the primary visual cortex of rats, using c-Fos biomarker as a reporter of light-evoked neuronal activity.