Photobactericidal polymers; the incorporation of crystal violet and nanogold into medical grade silicone
Photobactericidal polymers; the incorporation of crystal violet and nanogold into medical grade silicone
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DOI：
10.1039/c3ra42629g
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发表时间：
2013-01-01
期刊：
影响因子：
3.9
通讯作者：
Parkin, Ivan P.
中科院分区：
文献类型：
--
作者：
Noimark, Sacha;Bovis, Melissa;Parkin, Ivan P.
通过使用水和水-丙酮混合物的新型两步浸渍策略，将结晶紫和2纳米金纳米颗粒掺入医用级有机硅聚合物中。光学显微镜检查表明，优化后的聚合物在靠近聚合物表面的地方掺入了染料，在整个聚合物体中具有最小的染料封装。改性后的聚合物在37℃的水环境下是稳定的，结晶紫从聚合物中浸出到周围的水溶液中几乎可以忽略。将改性硅树脂暴露在低功率635 nm激光下可诱导表皮葡萄球菌和大肠杆菌致死性光敏。尽管所使用的激光与含结晶紫的硅胶样品的最大吸收不匹配，但就每能量剂量的细菌杀伤而言，致命光敏是最高的。此外，令人惊讶的是，一些统计上显著的暗杀也被注意到了。
Crystal violet and 2 nm gold nanoparticles were incorporated into medical grade silicone polymers by use of a novel two-step dipping strategy using water and water-acetone mixtures. Optical microscopic examination showed that the optimised polymer incorporated dye close to the polymer surface, with minimal dye encapsulation throughout the polymer bulk. The modified polymer was stable under aqueous conditions with negligible leaching of crystal violet from the polymer into surrounding aqueous solution at 37 degrees C. Exposure of the modified silicone to low power 635 nm laser light induced the lethal photosensitisation of both Staphylococcus epidermidis and Escherichia coli. Despite the laser used not matching the absorption maximum of the crystal violet-containing silicone samples, the lethal photosensitisation was the highest reported, in terms of bacterial kill per energy dose. Furthermore, surprisingly, some statistically significant dark kill was also noted.