P-AMINOPHENYL PHOSPHATE - AN IMPROVED SUBSTRATE FOR ELECTROCHEMICAL ENZYME-IMMUNOASSAY
P-AMINOPHENYL PHOSPHATE - AN IMPROVED SUBSTRATE FOR ELECTROCHEMICAL ENZYME-IMMUNOASSAY
复制标题
DOI：
10.1016/s0003-2670(00)80440-7
复制
发表时间：
1988-11-15
影响因子：
6.2
通讯作者：
HEINEMAN, WR
中科院分区：
文献类型：
--
作者：
TANG, HT;LUNTE, CE;HEINEMAN, WR
描述了一种用于酶免疫分析与电化学检测的替代底物。碱性磷酸酶（EC.3.1.3.1）活性通过使用磷酸对氨基苯酯作为酶底物测定。酶产生的对-氨基苯酚在玻碳电极上通过液相色谱电化学检测安培检测。对氨基苯酚的氧化电位低于苯酚，以前使用的底物产品。对氨基苯酚的检出限为0.20pmol。地高辛的检测限为30 pg/ml，酶反应的孵育时间为5 min。
An alternative substrate is described for enzyme immunosaasay with electrochemical detection. Alkaline phosphatase (EC.3.1.3.1) activity is determined by usingp-aminophenyl phosphate as the enzyme substrate. Enzyme-generatedp-aminophenol is detected amperometrically at a glassy carbon electrody by liquid chromatography with electrochemical detection. The oxidation potential obtained for the detectionofp-aminophenol is lower than that for phenol, the previously used substrate product. The detection limit forp-aminophenol is 0.20pmol. A detection limit of 30 pg ml-1for digoxin and a 5-min incubationtime for the enzyme reaction were obtained with the new system.