Glomeruloid vascular structures in glioblastoma multiforme: An immunohistochemical and ultrastructural study
Glomeruloid vascular structures in glioblastoma multiforme: An immunohistochemical and ultrastructural study
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DOI：
10.3171/jns.1996.85.6.1078
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发表时间：
1996-12-01
影响因子：
4.1
通讯作者：
DoroviniZis, K
中科院分区：
文献类型：
--
作者：
Rojiani, AM;DoroviniZis, K
微血管增生和肾小球样血管结构是多形性胶质母细胞瘤（GEM）的重要组织病理学特征。参与这些结构形成的细胞的性质仍不清楚，这是本研究的主题。为了定义这些细胞交换，使用针对因子Vm相关抗原（FVIIIR：Ag）、α平滑肌肌动蛋白（α-SMA）和凝集素荆豆凝集素I型（UEA-I）的免疫组织化学标记。血管通道内衬细胞和大量参与肾小球样血管结构形成的增殖近腔细胞显示FVIIIR：Ag和UEA-I阳性细胞质染色。将近腔和腔细胞标记为α-SMA。超微结构上，肾小球样血管结构由局灶性闭塞的毛细血管组成，管腔狭窄。内皮细胞增生，大小和形状不一，重叠局灶性，并含有大量的胞浆丝。紧密连接结合在一起的相邻和重叠的内皮细胞，韦伯-帕拉德体，通常不存在于脑微血管中，存在于新形成的毛细血管中的数量增加。每个毛细血管袢被基膜包围，基膜包含不连续的周细胞层。这项研究表明，肾小球血管结构在GEM是复杂的聚集体的新形成的微通道内衬增生的内皮细胞，具有改变的形态表型，这些微通道的支持下，基底层和周细胞，是没有星形胶质细胞终足。
Microvascular proliferation and glomeruloid vascular structures are important histopathological features of glioblastoma multiforme (GEM). The nature of cells participating in the formation of these structures remains unclear and is the subject of this study. To define these cells barter, immunohistochemical markers directed against Factor Vm-related antigen (FVIIIR:Ag), alpha smooth-muscle actin (alpha-SMA), and the lectin Ulex europaeus agglutinin type I (UEA-I) were used. Cells lining the vascular channels and a large number of proliferating abluminal cells participating in glomeruloid vascular structure formation showed positive cytoplasmic staining for FVIIIR:Ag and UEA-I. Abluminal and luminal cells were variably labeled For alpha-SMA. Ultrastructurally, complex aggregates of focally anastomosing capillaries with narrow lumina composed thr glomeruloid vascular structure. Endothelial cells were hyperplastic, varied in size and shape, overlapped focally, and contained numerous cytoplasmic filaments. Tight junctions bound together adjacent and overlapping endothelial cells, Weibel-Palade bodies, usually absent from brain microvessels, were present in increased numbers in the newly formed capillaries. Each capillary loop was surrounded by basal lamina encompassing a discontinuous layer of pericytes. This study indicates that glomeruloid vascular structures in GEM are complex aggregates of newly formed microchannels lined with hyperplastic endothelial cells that have an altered morphological phenotype and that these microchannels channels are supported by basal lamina and pericytes and are devoid of astrocytic end-feet.