Key Role of Phosphoenolpyruvate in the Regulation of Glycolysis-Gluconeogenesis in Thermus thermophilus HB 8
Key Role of Phosphoenolpyruvate in the Regulation of Glycolysis-Gluconeogenesis in Thermus thermophilus HB 8
复制标题
磷酸烯醇丙酮酸在嗜热栖热菌 HB 8 糖酵解-糖异生调节中的关键作用
DOI：
10.1271/bbb1961.43.537
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
K. Imahori
中科院分区：
文献类型：
--
作者：
F. Yoshizaki;K. Imahori
The in vivo concentrations of metabolites and the level of enzymes were measured to elucidate the regulatory mechanism by phosphoenolpyruvate of glycolysis-gluconeogenesis in an extreme thermophile, Thermus thermophilus HB8. Glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate, and fructose 1, 6-bisphosphate were low, and phosphoenol-pyruvate was high in the cells growing on polypeptone-yeast extract (PY cells). On the contrary, phosphoenolpyruvate was low and hexose phosphates were high in the cells growing on glucose-polypeptone-yeast extract (GPY cells). The difference in the values of adenylate energy charge was not observed between PY and GPY cells. Among various enzymes, phosphofructokinase and fructose 1, 6-bisphosphatase level changed markedly; the former was high in GPY cells and the latter in PY cells. It is reasonably considered that PY cells may turn on gluconeogenesis and GPY cells on glycolysis. The in vivo regulatory functions of key enzymes, phosphofructokinase, fructose 1, 6-bisphosphatase, and pyruvate kinase, and the important roles of phosphoenolpyruvate and fructose 6-phosphate were discussed from these findings.
