Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.
Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.
复制标题
具有纳米级空间分辨率的染色体的多模式硬X射线成像。
DOI：
10.1038/srep20112
复制
发表时间：
2016-02-05
影响因子：
4.6
通讯作者：
Chu YS
中科院分区：
文献类型：
--
作者：
Yan H;Nazaretski E;Lauer K;Huang X;Wagner U;Rau C;Yusuf M;Robinson I;Kalbfleisch S;Li L;Bouet N;Zhou J;Conley R;Chu YS
我们开发了一种扫描硬X射线显微镜，使用一类新的X射线纳米聚焦光学称为多层劳厄透镜和成像的染色体与纳米级的空间分辨率。硬X射线的上级穿透能力、对元素组成的高灵敏度、高空间分辨率和定量分析的组合创造了具有其他显微镜技术无法提供的能力的独特工具。使用这种显微镜，我们同时获得吸收，相位，和荧光对比图像的Pt染色的人类染色体样本。显微镜的高空间分辨率及其多模态成像能力使我们能够在不切片的情况下观察厚染色体的内部超微结构。
We developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray’s superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.