Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants
Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants
复制标题
单纯疱疹病毒 (HSV) DNA 的解剖。
作者：
L. Morse;L. Pereira;B. Roizman;P. Schaffer
在早期的一篇论文(Morse等人，J.Virol 24：231-248,1977)中，我们报道了26个单纯疱疹病毒1型(HSV-1)X HSV-2重组体的DNA序列的来源，这些重组体的DNA至少有5个限制性内切酶。本报告介绍了由重组子及其亲本HSV-1和HSV-2指定的多肽。我们已经鉴定出(I)相应的HSV-1和HSV-2多肽，分子量在20,000到200,000以上，(Ii)经过快速翻译后加工的多肽，以及(Iii)表观分子量在典型内变化的多肽。通过比较多肽与重组子DNA序列的分离模式，我们将26个多肽和几种病毒功能的模板定位在HSV DNA的物理图谱上。这些数据表明：(1)单纯疱疹病毒DNA L和S组分末端的α多肽图谱。尽管αICP27完全位于L组分的重复区域内，但αICP4的模板可能仅部分位于S组分的重复序列内。值得注意的是，发现感染了S成分中同时包含HSV-1和HSV-2DNA序列的重组体的细胞产生了HSV-1和HSV-2的α-ICP4。(Ii)指定β和伽马多肽的模板映射在L成分中，并且似乎是随机分布的。(Iii)在L成分中定位胸苷激酶和对膦乙酸的抗药性。此外，我们还利用了单纯疱疹病毒2型感染对宿主蛋白合成的快速抑制特性和合胞体斑块的形态，将负责这些功能的模板(S)也定位在L成分中。对HSV DNA中模板排列的意义进行了讨论。
In an earlier paper (Morse et al., J. Virol 24:231--248, 1977) we reported on the provenance of the DNA sequences in 26 herpes simplex virus type 1 (HSV-1) X HSV-2 recombinants as determined from analyses of their DNAs with at least five restriction endonucleases. This report deals with the polypeptides specified by the recombinants and by their HSV-1 and HSV-2 parents. We have identified (i) the corresponding HSV-1 and HSV-2 polypeptides with molecular weights ranging from 20,000 to more than 200,000, (ii) the polypeptides that undergo rapid post-translational processing, and (iii) polypeptides that vary intratypically in apparent molecular weight. By comparing the segregation patterns of the polypeptides with those of the DNA sequence of the recombinants, we have mapped the templates specifying 26 polypeptides and several viral functions on the physical map of HSV DNA. The data show the following: (i) alpha polypeptides map at the termini of the L and S components of the HSV DNA. Although alpha ICP 27 maps entirely within the reiterated region of the L component, the template for alpha ICP 4 may lie only in part within the reiterated sequences of the S component. Of note is the finding that cells infected with a recombinant that contains both HSV-1 and HSV-2 DNA sequences in the S component produced alpha ICP 4 of both HSV-1 and HSV-2. (ii) Templates specifying beta and gamma polypeptides map in the L component and appear to be randomly distributed. (iii) Thymidine kinase and resistance to phosphonoacetic acid mapped in the L component. In addition, we have taken advantage of the rapid inhibition of host protein synthesis characteristic of HSV-2 infections and syncytial plaque morphology to also map the template(s) responsible for these functions in the L component. The implications of the template arrangement in HSV DNA are discussed.