Gene expression analysis during the conversion from a viable but nonculturable to culturable state in Vibrio cholerae
Gene expression analysis during the conversion from a viable but nonculturable to culturable state in Vibrio cholerae
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DOI：
10.1016/j.gene.2023.147289
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发表时间：
2023-02-24
期刊：
影响因子：
3.5
通讯作者：
Hamabata，Takashi
中科院分区：
文献类型：
--
作者：
Ayibieke，Alafate;Nishiyama，Ayae;Hamabata，Takashi
我们以前报道过霍乱弧菌在活的但不可培养（VBNC）状态下可以通过过氧化氢酶处理转化为可培养状态。这一发现使我们能够开发一种检测系统来观察从VBNC转化为可培养的inV.霍乱菌的时间过程。补充过氧化氢酶后，早在2 h， VBNC细胞就开始向可培养细胞转化。采用RNA芯片技术分析过氧化氢酶作用下VBNC细胞的基因表达。过氧化氢酶暴露6 h后，许多核糖体DNA基因受到刺激，表明转换驱动信号在6 h之前就开始了。关注细胞增殖前的一段时间，我们发现16个基因可能参与了inV.霍乱的转换机制，它们在过氧化氢酶加入后的2 h和4 h表达增强。这些上调的基因包括噬菌体休克蛋白（pspA、B和c）、备选sigma因子（rpoE）及其负调节因子（rseA）、cowc末端结构域含蛋白、损伤诱导解旋酶（dinG）、疟原虫毒素分泌蛋白epsm、hth型转录调节因子（iscR）、机械敏感离子通道家族蛋白、蒽环类合成酶组分I、果糖特异性IIBC组分、钼进口atp结合蛋白（modC）、LysE家族转运子、假定的有机氢过氧化物抗性蛋白，和一种假定的蛋白质。本研究确定了参与过氧化氢酶诱导的v转化的基因。并为转化过程中涉及的机制提供了有价值的见解。
We previously reported thatVibrio choleraein a viable but non-culturable (VBNC) state can be converted to a culturable state by treatment with catalase. This finding enabled us to develop an assay system to observe the time course of the conversion from VBNC to culturable inV. cholerae.VBNC cells began to convert to culturable cells as early as 2 h after catalase supplementation. Gene expression in VBNC cells during catalase treatment was analyzed using RNA microarray. Many ribosomal DNA genes were stimulated 6 h post catalase exposure, suggesting that the conversion-driving signal started prior to 6 h. Focusing on the period prior to cell proliferation, we found that 16 genes might be involved in the conversion mechanism inV. cholerae,and they showed enhanced expression at 2 h and 4 h after catalase addition. These upregulated genes included phage shock proteins (pspA,B, andC), alternative sigma factor (rpoE) and its negative regulator (rseA),cobWC terminal domain-containing protein, damage-inducible helicase (dinG), cholerae toxin secretion proteinepsM, HTH-type transcription regulator (iscR), mechanosensitive ion channel family protein, anthranilate synthase component I, fructose-specific IIBC component, molybdenum import ATP-binding protein (modC), LysE family translocator, putative organic hydroperoxide resistance protein, and a hypothetical protein. This study identified genes involved in the catalase-induced conversion ofV. choleraeVBNC cells to a culturable state and provided valuable insights into the mechanisms involved in the conversion process.