Polymerization of vector DNA after transfection into hamster fibroblast cells.
Polymerization of vector DNA after transfection into hamster fibroblast cells.
复制标题
转染仓鼠成纤维细胞后载体 DNA 聚合。
DOI：
10.1016/0006-291x(83)91191-9
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发表时间：
1983
影响因子：
3.1
通讯作者：
Lee,AS
中科院分区：
文献类型：
--
作者：
Wang,ML;Lee,AS
我们用两个克隆的、可选择的标记物转化了仓鼠肺成纤维细胞，这两个标记物是广泛使用的pSV2gpt载体和pNEO3， pNEO3赋予了对新霉素的抗性。从数百个转化子的个体和集合组中提取的DNA分析表明，在这些无载体DNA的转化实验中，载体DNA以串联形式存在于转化子中。集成向量的拷贝数可以从几个到几百个不等。此外，在pSV2gpt的情况下，转换效率提高了十倍。
We have transformed hamsters lung fibroblasts with two cloned, selectable markers, the widely used pSV2gpt vector and pNEO3, which confers resistance to neomycin. Analysis of DNA extracted from both individual and pooled groups of several hundred transformants reveal that in these carrier DNA-free transformation experiments, the vector DNA are found in concatenate forms in the transformants. The copy number of the integrated vectors can vary from a few to several hundred. In addition, the efficiency of transformation is ten times higher in the case of pSV2gpt.