GmAGL1, a MADS-Box Gene from Soybean, Is Involved in Floral Organ Identity and Fruit Dehiscence.
GmAGL1, a MADS-Box Gene from Soybean, Is Involved in Floral Organ Identity and Fruit Dehiscence.
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GmAGL1 是一种来自大豆的 MADS-Box 基因，参与花器官识别和果实开裂
DOI：
10.3389/fpls.2017.00175
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发表时间：
2017
影响因子：
5.6
通讯作者：
Huang F
中科院分区：
文献类型：
--
作者：
Chi Y;Wang T;Xu G;Yang H;Zeng X;Shen Y;Yu D;Huang F
MADS结构域蛋白是重要的转录因子，参与植物生殖发育的许多方面。本研究从大豆花中克隆了MADS-box基因Glycine max AGAMOUS-LIKE 1（GmAGL 1）。GmAGL 1基因在大豆不同发育时期的花和豆荚中均有表达，在心皮中表达量较高。GmAGL 1是一种核定位转录因子，可与大豆花中的SEP样蛋白直接相互作用。GmAGL 1的异位过表达导致拟南芥中没有花瓣。此外，在种子完全成熟之前开裂的35 S：GmAGL 1拟南芥果实中观察到了瓣的形态变化。发现GmAGL 1足以激活拟南芥ALC、IND、STK、SEP 1和SEP 3的表达。因此，我们的数据表明，GmAGL 1可能发挥重要作用，在花器官的身份和果实开裂。
MADS-domain proteins are important transcription factors involved in many aspects of plant reproductive development. In this study, a MADS-box gene, Glycine max AGAMOUS-LIKE1 (GmAGL1), was isolated from soybean flower. The transcript of GmAGL1 was expressed in flowers and pods of different stages in soybean and was highly expressed in carpels. GmAGL1 is a nucleus-localized transcription factor and can interact directly with SEP-like proteins in soybean flowers. Ectopic overexpression of GmAGL1 resulted in the absence of petals in Arabidopsis. Moreover, morphological changes in the valves were observed in 35S:GmAGL1 Arabidopsis fruits that dehisced before the seeds reached full maturity. GmAGL1 was found to be sufficient to activate the expression of Arabidopsis ALC, IND, STK, SEP1, and SEP3. Therefore, our data suggest that GmAGL1 may play important roles in both floral organ identity and fruit dehiscence.