Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors.
Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors.
复制标题
单子叶植物中病毒介导的蛋白质过表达（VOX）可鉴定真菌效应子并对其进行功能表征。
DOI：
10.1007/978-1-0716-2449-4_7
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kanyuka K
中科院分区：
文献类型：
--
作者：
Kanyuka K
病原体成功定殖植物的重要武器之一是分泌的小效应蛋白，它可以发挥多种功能，从抑制植物先天免疫到操纵植物生理以促进疾病。另一方面，植物进化出抗病基因，识别一些效应物或无毒蛋白。Avr蛋白的鉴定和其他效应物的作用机制的理解都是植物与病原体分子相互作用领域的重要研究领域，因为这些知识对于开发新的有效的病原体控制措施至关重要。为了能够对效应器进行功能分析，希望能够在宿主植物中容易地过表达它们。本文详细描述了利用大麦条纹花叶病毒（BSMV）和狐尾花叶病毒（FoMV）衍生载体在小麦和其他单子叶植物中瞬时效应物过表达的实验方案。这种功能基因组学工具，更广为人知的名字是VOX（病毒介导的蛋白过表达），快速、相对简单且便宜。
One of the important armories that pathogens utilize to successfully colonize the plants is small secreted effector proteins, which could perform a variety of functions from suppression of plant innate immunity to manipulation of plant physiology in favor of the disease. Plants, on the other hand, evolved disease resistance genes that recognize some of the effectors or avirulence (Avr) proteins. Both, identification of the Avr proteins and understanding of the mechanisms of action of other effectors, are important areas of research in the molecular plant-pathogen interactions field as this knowledge is critical for the development of new effective pathogen control measures. To enable functional analysis of the effectors, it is desirable to be able to overexpress them readily in the host plants. Here we describe detailed experimental protocols for transient effector overexpression in wheat and other monocots using binaryBarley stripe mosaic virus(BSMV)- andFoxtail mosaic virus(FoMV)-derived vectors. This functional genomics tool, better known as VOX (Virus-mediated protein OvereXpression), is rapid and relatively simple and inexpensive.