Edaphobaculum flavum gen. nov., sp nov., a member of family Chitinophagaceae, isolated from grassland soil
Edaphobaculum flavum gen. nov., sp nov., a member of family Chitinophagaceae, isolated from grassland soil
复制标题
Edaphobaculum flavum gen.
DOI：
10.1099/ijsem.0.002316
复制
发表时间：
2017-11-01
影响因子：
2.8
通讯作者：
Wang, Gejiao
中科院分区：
文献类型：
--
作者：
Cao, Min;Huang, Jing;Wang, Gejiao
从内蒙古鄂尔多斯草原土壤中分离到一株黄色、革兰氏阴性、不能运动、杆状、需氧严格的细菌，命名为1-116(T)，编号为PR中国。基于16S rRNA基因的系统发育分析表明，菌株1-116(T)与朝鲜太白杆菌THG-DT86(T)和立枯黄杆菌3-3(T)的相似性最高，分别为90.1%和90.0%，而与其他几丁虫类型菌株的相似性均低于90.0%。菌株1-116(T)在16-33℃(最适28℃)、pH 6.0-9.0(最适7.0-8.0)和0-0.5%氯化钠(w/v；不含氯化钠)条件下生长。合成了一种柔红素型色素。DNA G+C含量为43.2mol%，唯一存在的苯二酚为孟喹酮-7。唯一检测到的多胺为高聚亚胺[30.7mU·mol(g干重)(-1)]，主要脂肪酸为iso-C-15(：)(0)(20.8%)、iso-C-15：(1)G(25.1%)、总和特征4(anteisC-17：1B和/或iso-C-17：1I；13.8%)和iso-C-17：03-OH(13.2%)。主要的极性脂类是磷脂酰乙醇胺、三种未知的脂类、一种未知的氨基磷脂和一种未知的糖脂。在多相分析的基础上，菌株1-116(T)代表了几丁聚糖科的一个新属和新种，命名为黄色埃达夫杆菌。Nov.，sp.Nov.，提出了一种新方法。模式菌株为1-116(T)(=CCTCC AB 2017054=KCTC 52843)。
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116(T), was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116(T) was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86(T) (90.1 %) and Flavihumibacter solisivae 3-3(T) (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %. Strain 1-116(T) grew at 16-33 degrees C (optimum 28 degrees C), pH 6.0-9.0 (optimum 7.0-8.0) and 0-0.5% NaCl (w/v; optimum without NaCl). A flexirubin-type pigment was present. The DNA G+C content was 43.2 mol% and the only quinone present was menaquinone-7. The only polyamine detected was sym-homospermidine [30.7 mu mol (g dry weight)(-1)] and the predominant fatty acids were iso-C-15 (:) (0) (20.8 %), iso-C-15 : (1) G (25.1 %), summed feature 4 (anteiso-C-17 : 1 B and/or iso-C-17 : 1 I; 13.8 %) and iso-C-17 : 0 3-OH (13.2 %). The major polar lipids were phosphatidylethanolamine, three unidentified lipids, an unidentified aminophospholipid and an unidentified glycolipid. On the basis of the polyphasic analyses, strain 1-116(T) represents a novel genus and species in the family Chitinophagaceae, for which the name Edaphobaculum flavum gen. nov., sp. nov., is proposed. The type strain of Edaphobaculum flavum is 1-116(T) (=CCTCC AB 2017054=KCTC 52843).