Expression patterns of genes and enzymes involved in sugar catabolism in industrial Saccharomyces cerevisiae strains displaying novel fermentation characteristics
Expression patterns of genes and enzymes involved in sugar catabolism in industrial Saccharomyces cerevisiae strains displaying novel fermentation characteristics
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DOI：
10.1002/j.2050-0416.2002.tb00557.x
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Jiranek, V
中科院分区：
文献类型：
--
作者：
Meneses, FJ;Jiranek, V
利用麦芽糖或蔗糖的选择9酿造，烘焙或实验室菌株的酿酒酵母是可抑制的，组成或不存在。总体发酵速率与最大麦芽糖比转运速率（R-2 = 0.79）具有良好的相关性，但与最大麦芽糖酶活性（R-2 = 0.34）的相关性较差，这意味着麦芽糖的转运而不是水解是决定发酵性能的限速步骤。研究了菌株之间在发酵动力学方面的差异的遗传基础。发现所有菌株都具有用MAL 11（AGT 1）、MAL 31通透酶基因和SUC 2转化酶基因的探针可检测的基因组序列，然而，这些和其它MAL基因的存在位点和表达模式变化很大。从糖利用和基因表达模式的比较，组成型麦芽糖利用在菌株NCYC 1681中最好的解释AGT 1的表达。然而，转录激活因子（MALx 3）表达模式的变化并不反映AGT 1的这种表达。模式的蔗糖利用率预测不佳的动力学SUC 2基因的表达，表明其他SUC位点可能是更重要的。
The utilisation of maltose or sucrose by a selection of nine brewing, baking or laboratory strains of Saccharomyces cerevisiae was either repressible, constitutive or absent. Overall fermentation rate showed a good correlation with maximum specific maltose transport rate (R-2 = 0.79), but a poor correlation with maximum maltase activity (R-2 = 0.34), implying that transport rather than hydrolysis of maltose was a rate-limiting step determining fermentation performance. The genetic basis for differences seen between the strains in terms of fermentation kinetics was investigated. All strains were found to possess genomic sequences detectable with probes for the MAL11 (AGT1), MAL31 permease genes and the SUC2 invertase gene, however, the loci present and the pattern of expression of these and other MAL genes varied widely. From comparisons of sugar utilisation and gene expression patterns, constitutive maltose utilisation in strain NCYC 1681 was best explained by AGT1 expression. Such expression of AGT1 was not, however, mirrored by changes in the pattern of expression of the transcriptional activator(s) (MALx3). Patterns of sucrose utilisation were poorly predicted by the kinetics of SUC2 gene expression, indicating that other SUC loci may be of greater importance.