Influence of different divalent metal ions on the properties of alginate microcapsules and microencapsulated cells
Influence of different divalent metal ions on the properties of alginate microcapsules and microencapsulated cells
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DOI：
10.1007/s10971-013-3051-4
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发表时间：
2013-04
影响因子：
2.5
通讯作者：
Yuangang Liu;Yuesheng Tong;Shi-Bin Wang;Qiongjia Deng;A. Chen
中科院分区：
文献类型：
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作者：
Yuangang Liu;Yuesheng Tong;Shi-Bin Wang;Qiongjia Deng;A. Chen
选择不同的二价金属离子(Ba2+、Sr2+、Ca2+、Zn2+)作为交联剂。对材料的力学性能、细胞相容性、组织相容性、细胞增殖和长期培养进行了研究。制得的微胶囊球形度好，表面光滑，粒径分布在300~400nμm之间，具有较好的机械强度。所有膜的截留分子量均在24~67 kDa之间。所有微囊均无细胞毒性。经腹腔移植后，回收的微囊仍保持良好的机械强度和形态，未见纤维化或巨噬细胞聚集现象。微囊化的PC12细胞在微囊破裂后重新培养后无明显变化。72小时后，不同微囊的细胞活性大小顺序为：空白对照细胞>；Sr2+>；钙离子>；Ba2+>；体外培养9周后，细胞存活率达80%以上。本论文对海藻酸盐/壳聚糖包埋药物或细胞微囊的基础研究和临床应用具有一定的科学意义。
Different divalent metal ions (Ba2+, Sr2+, Ca2+, Zn2+) were selected as crosslinkers. The mechanical properties, cytocompatibility, histocompatibility, cell proliferation and long-term cultivation were investigated. The resulting microcapsules had good sphericity, smooth surface and particle size distribution of 300–400 μm. Sr2+microcapsules exhibited a better mechanical strength. The molecular weights cut-off of all membranes were between 24 and 67 kDa. All microcapsules had no cytotoxicity. After intraperitoneal transplantation, the recovered microcapsules still maintained good mechanical strength and morphology with no fibrosis or macrophage aggregation phenomena. The microencapsulated PC12 cells showed no significant variation after recultivation following microcapsule breaking. The cell activity sequence of different microcapsules after 72 h was as follows: bare control cell >Sr2+> Ca2+> Ba2+> Zn2+. After 9 weeks’ in vitro culture, the cell survival rate was higher than 80 %. This paper will be of scientific interests for the basic research and clinical application of alginate/chitosan microcapsules embedded with drugs or cells.