Frequency and typing of Propionibacterium acnes in prostate tissue obtained from men with and without prostate cancer
Frequency and typing of Propionibacterium acnes in prostate tissue obtained from men with and without prostate cancer
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DOI：
10.1186/s13027-016-0074-9
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发表时间：
2016-06-09
影响因子：
3.7
通讯作者：
Andren, Ove
中科院分区：
文献类型：
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作者：
Davidsson, Sabina;Molling, Paula;Andren, Ove
背景资料：前列腺癌是西方国家男性中最常见的癌症，但其确切的致病机制仍在很大程度上未知。感染性病因学和感染诱导的炎症已被认为在前列腺癌发生中起作用，并且痤疮丙酸杆菌已被报道为前列腺组织中最普遍的微生物。我们调查的频率和类型的痤疮丙酸杆菌分离的前列腺组织样本的男性前列腺癌和对照patients没有diseases.Methods：我们包括100例和50名对照在这项研究中。病例为诊断为前列腺癌并接受根治性前列腺切除术的男性，对照组为接受膀胱癌手术但无任何前列腺癌组织学发现的男性。在手术时从每个患者的前列腺中取出的六个活组织切片用于培养和进一步表征痤疮丙酸杆菌。结果：结果显示，痤疮丙酸杆菌在患有前列腺癌的男性中比在对照组中更常见，在60%的病例中培养出细菌，而在对照组中培养出细菌的比例为26%（p = 0.001）。在多变量分析中，在调整年龄、手术日历年和吸烟状况后，患有痤疮丙酸杆菌的男性患前列腺癌的几率增加了4倍（OR：4.46; 95% CI：1.93-11.26）。为了进一步支持痤疮丙酸杆菌感染作为前列腺癌发展的促成因素的生物学可接受性，我们随后进行了基于细胞的实验。将痤疮丙酸杆菌分离物与前列腺细胞系PNT 1A共培养。结论：本研究为痤疮丙酸杆菌在前列腺癌发生发展中的作用提供了进一步的证据。
Background: Prostate cancer is the most common cancer among men in Western countries but the exact pathogenic mechanism of the disease is still largely unknown. An infectious etiology and infection-induced inflammation has been suggested to play a role in prostate carcinogenesis and Propionibacterium acnes has been reported as the most prevalent microorganism in prostatic tissue. We investigated the frequency and types of P. acnes isolated from prostate tissue samples from men with prostate cancer and from control patients without the disease.Methods: We included 100 cases and 50 controls in this study. Cases were men diagnosed with prostate cancer undergoing radical prostatectomy and controls were men undergoing surgery for bladder cancer without any histological findings of prostate cancer. Six biopsies taken from each patient's prostate gland at the time of surgery were used for cultivation and further characterization of P. acnes.Results: The results revealed that P. acnes was more common in men with prostate carcinoma than in controls, with the bacteria cultured in 60 % of the cases vs. 26 % of the controls (p = 0.001). In multivariable analyses, men with P. acnes had a 4-fold increase in odds of a prostate cancer diagnosis after adjustment for age, calendar year of surgery and smoking status (OR: 4.46; 95 % CI: 1.93-11.26). To further support the biologic plausibility for a P. acnes infection as a contributing factor in prostate cancer development, we subsequently conducted cell-based experiments. P. acnes-isolates were co-cultured with the prostate cell line PNT1A. An increased cell proliferation and cytokine/chemokine secretion in infected cells was observed.Conclusion: The present study provides further evidence for a role of P. acnes in prostate cancer development.