EXPRESSION IN MAMMALIAN-CELLS OF A GENE FROM STREPTOMYCES-ALBONIGER CONFERRING PUROMYCIN RESISTANCE
EXPRESSION IN MAMMALIAN-CELLS OF A GENE FROM STREPTOMYCES-ALBONIGER CONFERRING PUROMYCIN RESISTANCE
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DOI：
10.1093/nar/14.11.4617
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发表时间：
1986-06-11
影响因子：
14.9
通讯作者：
JIMENEZ, A
中科院分区：
文献类型：
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作者：
VARA, JA;PORTELA, A;JIMENEZ, A
在哺乳动物细胞-大肠杆菌穿梭载体SV40早期启动子旁克隆了编码白色链霉菌嘌呤霉素n -乙酰转移酶的基因。当这种结构被引入到VERO细胞时，它表达了相关的酶活性。此外，嘌呤霉素n -乙酰转移酶基因已被用作选择能够在抗生素存在下生长的转化哺乳动物细胞的显性标记。
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shutle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.