HETEROGENEITY OF NORMAL HUMAN-DIPLOID FIBROBLASTS - ISOLATION AND CHARACTERIZATION OF ONE PHENOTYPE
HETEROGENEITY OF NORMAL HUMAN-DIPLOID FIBROBLASTS - ISOLATION AND CHARACTERIZATION OF ONE PHENOTYPE
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DOI：
10.1126/science.6691142
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
NARAYANAN, AS
中科院分区：
文献类型：
--
作者：
BORDIN, S;PAGE, RC;NARAYANAN, AS
人二倍体成纤维细胞的培养含有细胞，通过启动DNA合成和生长来响应补体C1的暴露。这些细胞的质膜具有C1的C1q亚组分的特异性结合位点。利用荧光激活的细胞分选器分离出一组对C1q具有高亲和力的细胞亚群，并在体外多次重复后研究这些高亲和力细胞的生长和合成活性。这些细胞合成DNA，生长速度比亲本培养和低亲和细胞快，产生的蛋白质是亲本培养和低亲和细胞的2-3倍。它们总蛋白质合成活性的40%左右直接用于胶原蛋白的生成，其中III型和V型胶原蛋白的生成比例异常高。这些特性和细胞对C1q的高亲和力在至少6次细胞转移中保持不变。这种表型具有愈合伤口和炎症组织中成纤维细胞的特性。
Cultures of human diploid fibroblasts contain cells that respond to exposure to the complement C1 by initiating DNA synthesis and growth. The plasma membranes of these cells have specific binding sites for the C1q subcomponent of C1. A fluorescence-activated cell sorter was used to isolate a subset of cells with a high affinity for C1q, and the growth and synthesis activities of these high-affinity cells were studied after numerous replications in vitro. These cells synthesize DNA and grow faster than the parent cultures and low-affinity cells, and they produce 2-3 times as much protein. About 40% of their total protein synthesis activity is directed to collagen production, unusually high proportions of collagen types III and V being produced. These properties and the high affinity of the cells for C1q are retained for at least 6 cell transfers. This phenotype has the properties expected of fibroblasts in healing wounds and inflamed tissues.