CHANGE IN ELECTROPHORETIC MOBILITY ASSOCIATED WITH SHAPE CHANGE OF HUMAN-BLOOD PLATELETS
CHANGE IN ELECTROPHORETIC MOBILITY ASSOCIATED WITH SHAPE CHANGE OF HUMAN-BLOOD PLATELETS
复制标题
DOI：
10.1098/rspb.1977.0052
复制
发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
MICHAL, F
中科院分区：
文献类型：
--
作者：
BOISSEAU, MR;LORIENT, MF;MICHAL, F
所有生理pH血小板，像其他细胞一样，在表面上具有过量的负电荷，这决定了它们的电泳迁移率（Dallan & Vassar 1966;汉普顿& Mitchell 1974）。这些负电荷的大部分是由于唾液酸结合到膜糖蛋白（麦道夫，Ebbe和Baldini 1964）。通过神经氨酸酶从血小板表面除去唾液酸会降低血小板的电泳迁移率（Bray &亚历山大1969）。最近已经表明，血小板在ADP或5-羟色胺聚集之前的快速形状变化与神经氨酸酶可去除的唾液酸的增加有关（Motamed，Michal & Born 1976）。如果这种唾液酸的增加是在血小板表面上，而血小板表面决定了它们的电泳迁移率，那么它应该表现为这种迁移率的增加。本文表明，当ADP引起形状变化时，迁移率增加。
All physiological pH platelets, like other cells, have an excess of negative electric charges on the surface which determine their electrophoretic mobility (Seaman & Vassar 1966; Hampton & Mitchell 1974). The greater part of these negative charges are due to sialic acids bound to membrane glycoproteins (Madoff, Ebbe & Baldini 1964). Removal of sialic acids from the platelet surface by neuraminidase diminishes the electrophoretic mobility of platelets (Bray & Alexander 1969). It has been shown recently that the rapid shape change of platelets which precedes their aggregation by ADP or 5-hydroxytryptamine is associated with an increase in sialic acids removable by neuraminidase (Motamed, Michal & Born 1976). If this increase in sialic acids were on the platelet surface which determines their electrophoretic mobility, it should show itself as an increase in that mobility. This paper shows that the mobility increases when the shape change is induced by ADP.