METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST
METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST
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DOI：
10.1016/0165-1161(75)90046-1
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
YAMASAKI, E
中科院分区：
文献类型：
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作者：
AMES, BN;MCCANN, J;YAMASAKI, E
本文介绍了沙门氏菌/微粒体试验作为诱变筛选系统的一般方法。所述方法包括标准平板试验、细菌试验菌株的使用和储存、肝匀浆（S/sub 9/）的制备和使用以及诱导大鼠微粒体酶活性升高的方法。该测试系统的应用，筛选大量的化合物，和测试结果的解释进行了讨论。
We describe in this paper the general methods for using the Salmonella/microsome test as a mutagenesis screening system. The methods described include the standard plate test, the use and storage of the bacterial tester strains, preparation and use of the liver homogenates (S/sub 9/), and the methods of inducing the rats for elevated microsomal enzyme activity. The application of this test system to screening large numbers of compounds, and the interpretation of test results is also discussed.