Effects of low concentrations of ethanol on the fluidity of spin-labeled erythrocyte and brain membranes.
Effects of low concentrations of ethanol on the fluidity of spin-labeled erythrocyte and brain membranes.
复制标题
低浓度乙醇对自旋标记红细胞和脑膜流动性的影响。
DOI：
--
复制
发表时间：
1977
影响因子：
3.6
通讯作者：
Dora B. Goldstein
中科院分区：
文献类型：
--
作者：
J. H. Chin;Dora B. Goldstein
乙醇对 37° 膜流动性的影响已通过灵敏电子顺磁共振技术进行了评估。 DBA/2J 小鼠的红细胞和脑膜用 5-羟基硬脂酸（5-酮硬脂酸的 N-氧基-49,49-二甲基恶唑烷衍生物）进行旋转标记。通过确定有序参数 S（膜流动性指数），从 EPR 谱中测量自旋标记的分子运动。红细胞膜和突触体膜的流动性均大于髓磷脂，但小于线粒体膜。体外添加低浓度（0.02 或 0.04 M）乙醇可增加红细胞、线粒体和突触体膜的流动性。在除髓磷脂外的所有膜中，乙醇的这种流化作用均与剂量相关，最高可达 0.35 M。这些数据表明，非致死浓度的乙醇可能会增加体内膜的流动性。
The effects of ethanol on membrane fluidity at 37° have been assessed by a sensitive electron paramagnetic resonance technique. Erythrocyte and brain membranes from DBA/2J mice were spin-labeled with 5-doxylstearic acid ( N -oxyl-49,49-dimethyloxazolidine derivative of 5-ketostearic acid). The molecular motion of the spin label was measured from the EPR spectrum by determining the order parameter S , an index of membrane fluidity. The fluidity of both erythrocyte and synaptosomal membranes was greater than that of myelin but less than that of mitochondrial membranes. The addition of low concentrations (0.02 or 0.04 M) of ethanol in vitro increased fluidity in erythrocyte, mitochondrial, and synaptosomal membranes. This fluidizing effect of ethanol was dose-related up to 0.35 M in all the membranes except myelin. These data suggest that nonlethal concentrations of ethanol may increase membrane fluidity in vivo .