Laboratory Bioassays with Three Different Substrates to Test the Efficacy of Insecticides against Various Stages of Drosophila suzukii (Diptera: Drosophilidae).
Laboratory Bioassays with Three Different Substrates to Test the Efficacy of Insecticides against Various Stages of Drosophila suzukii (Diptera: Drosophilidae).
复制标题
具有三种不同底物的实验室生物测定，以测试杀虫剂对苏木果果蝇（Diptera：果蝇）各个阶段的疗效。
DOI：
10.1093/jisesa/iew100
复制
发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Reineke A
中科院分区：
文献类型：
--
作者：
Pavlova AK;Dahlmann M;Hauck M;Reineke A
点翅果蝇（Drosophila suzukii Matsumura，双翅目：果蝇科）在全球范围内的快速传播和多食性特性要求采取有效的选择性控制策略，以防止各种水果作物的严重经济损失。使用杀虫剂是管理这种入侵害虫的一种选择。杀虫剂的药效通常首先在实验室生物测定中进行评估，这与D.此外，在生物测定中使用的水果通常在幼虫达到成虫阶段之前就开始腐烂和溶解。在这里，我们报告了使用三种不同类型基质的实验室生物测定，以便彻底筛选杀虫剂对D的潜在影响。Suzukii卵、幼虫和成虫。我们的生物测定的适用性进行了验证，在四个生物杀虫剂和一个合成杀虫剂对不同发育阶段的D。铃木。水苹果汁琼脂作为生物测定基板允许鸡蛋计数和观察幼虫发育，由于其透明度，而苹果营养培养基允许完全变态。在生物测定中使用葡萄浆果使得有可能评估水果表面上存在的杀虫剂对产卵和幼虫从卵中孵化的影响。在以啶虫脒、多杀菌素和天然除虫菊酯为活性成分的三种不同生物测定中，如果在卵沉积之前施用，则可以控制Suzukii。如果在产卵之前或之后用含有印苦楝素A的杀虫剂处理生物测定培养基，则成蝇数量显着减少。
Rapid worldwide spread and polyphagous nature of the spotted wing Drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae) calls for efficient and selective control strategies to prevent severe economic losses in various fruit crops. The use of insecticides is one option for management of this invasive pest insect. Efficacy of insecticides is usually assessed first in laboratory bioassays, which are compounded by the cryptic nature of D. suzukii larvae and the fact that fruits used in bioassays often start to rot and dissolve before larvae have reached the adult stage. Here, we report on laboratory bioassays using three different types of substrates allowing a thorough screening of insecticides for their potential effects against D. suzukii eggs, larvae and adults. Suitability of our bioassays was validated in an assessment of the efficacy of four bioinsecticides and one synthetic insecticide against various developmental stages of D. suzukii. Water-apple juice agar used as a bioassay substrate allowed egg counting and observation of larval development due to its transparency, while apple-nutrition medium allowed complete metamorphosis. Use of grape berries in bioassays made it possible to assess effects of an insecticide present on a fruit’s surface on oviposition and larval hatch from eggs. Insecticides tested in these three different bioassays with acetamiprid, spinosad or natural pyrethrins as active ingredients achieved a significant D. suzukii control if they were applied before egg deposition. Number of adult flies was significantly reduced if the bioassay medium was treated with an azadirachtin A containing insecticide both before or after egg deposition.