High-density mapping of single-molecule trajectories with photoactivated localization microscopy
High-density mapping of single-molecule trajectories with photoactivated localization microscopy
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DOI：
10.1038/nmeth.1176
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发表时间：
2008-02-01
期刊：
影响因子：
48
通讯作者：
Lippincott-Schwartz, Jennifer
中科院分区：
文献类型：
--
作者：
Manley, Suliana;Gillette, Jennifer M.;Lippincott-Schwartz, Jennifer
我们结合光激活定位显微镜（PALM）与活细胞单粒子跟踪，创建一个新的方法称为sptPALM。我们通过对膜蛋白Gag和VSVG进行成像，创建了单分子运动的空间分辨图，并获得了比传统单粒子跟踪多几个数量级的轨迹。通过探测分子的不同子集，sptPALM可以提供对膜中空间和时间异质性起源的洞察。
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.