A New Methodology for Quantitative LSPR Biosensing and Imaging
A New Methodology for Quantitative LSPR Biosensing and Imaging
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DOI：
10.1021/ac2023266
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发表时间：
2012-02-07
影响因子：
7.4
通讯作者：
Byers, Jeff M.
中科院分区：
文献类型：
--
作者：
Raphael, Marc P.;Christodoulides, Joseph A.;Byers, Jeff M.
提出了一种用于确定表面受体占据分数的局域表面等离子共振 (LSPR) 生物传感的新定量分析方法，以及用于结合事件时空映射的 LSPR 成像技术。使用电子束纳米光刻在玻璃盖玻片顶部制造 20 X 20 的金纳米结构阵列。使用单个生物素化阵列通过光谱确定作为时间函数的分数占用来测量中性抗生物素蛋白与表面的缔合动力学。通过再生相同的阵列，可以在对照样品和浓度范围从 1 μM 到 50 nM 的中性亲和素之间进行可靠的动力学比较。基于 CCD 的阵列图像与光谱测量同时拍摄，揭示了中性抗生物素蛋白与表面的结合，这通过大部分共振峰上的散射增强来证明。 LSPR成像技术的时间分辨率为200ms，空间分辨率为8μm(2)。
A new quantitative analysis methodology for localized surface plasmon resonance (LSPR) biosensing which determines surface-receptor fractional occupancy, as well as an LSPR imaging technique for the spatiotemporal mapping of binding events, is presented. Electron beam nanolithography was used to fabricate 20 X 20 arrays of gold nanostructures atop glass coverslips. A single biotinylated array was used to measure the association kinetics of neutravidin to the surface by spectroscopically determining the fractional occupancy as a function of time. By regenerating the same array, a reliable comparison of the kinetics could be made between control samples and neutravidin concentrations ranging from 1 mu M to SO nM. CCD-based imagery of the array, taken simultaneously with the spectroscopic measurements, reveals the binding of neutravidin to the surface as manifested by enhanced scattering over the majority of the resonance peak. The temporal resolution of the LSPR imaging technique was 200 ms and the spatial resolution was 8 mu m(2).