MicroRNA-122 Promotes Proliferation, Invasion and Migration of Renal Cell Carcinoma Cells Through the PI3K/Akt Signaling Pathway
MicroRNA-122 Promotes Proliferation, Invasion and Migration of Renal Cell Carcinoma Cells Through the PI3K/Akt Signaling Pathway
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DOI：
10.7314/apjcp.2013.14.9.5017
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Li, Yi
中科院分区：
文献类型：
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作者：
Lian, Ji-Hu;Wang, Wei-Hua;Li, Yi
目的：MicroRNAs （miRNAs）是一类非编码单链rna，主要通过结合靶mRNA的3'-非翻译区（3' utr）导致翻译阻滞和/或mRNA降解，在肾癌的发生和维持中起关键作用。本研究的目的是探讨miR-122在人肾细胞癌中的潜在作用。方法：采用qRT-PCR法检测miR-122的表达水平。通过MTT、集落形成、侵袭和迁移实验来探索miR-122在人肾细胞癌细胞中的潜在功能。结果：转染miR-122后，A498和786-O两种细胞的生长、侵袭和迁移均明显增加。进一步的实验表明，过表达miR-122导致磷酸化akt （Ser473）和磷酸化mTOR （Ser2448）的增加，进而激活mTOR靶点p70S6K和4E-BP1。结论：miR-122的上调可能通过激活PI3K/Akt信号通路在肾癌的进展中发挥重要作用，可能成为抗癌治疗的潜在分子靶点。
Objective: MicroRNAs (miRNAs) are a small class of non-coding, single-stranded RNAs with a critical role in genesis and maintenance of renal cancer mainly through binding to 3'-untranslated regions (3'UTR) of target mRNAs, which causes a block of translation and/or mRNA degradation. The aim of the present study was to investigate the potential effects of miR-122 in human renal cell carcinomas. Methods: The expression level of miR-122 was quantified by qRT-PCR. MTT, colony formation, invasion and migration assays were used to explore the potential functions of miR-122 in human renal cell carcinoma cells. Results: Cellular growth, invasion and migration in two A498 and 786-O cells were significantly increased after miR-122 transfection. Further experiments demonstrated that overexpression of miR-122 resulted in the increase of phospho-Akt (Ser473) and phospho-mTOR (Ser2448), then activation of mTOR targets, p70S6K and 4E-BP1. Conclusions: The up-regulation of miR-122 may play an important role in the progress of renal cancer through activating PI3K/Akt signal pathway and could be a potential molecular target for anti-cancer therapeutics.