Brain/gut peptides in fed and fasted rats.
Brain/gut peptides in fed and fasted rats.
复制标题
进食和禁食大鼠的脑/肠肽。
作者：
B. Zheng;J. Eng;R. S. Yalow
将 16 只禁食 5 天的成年 Sprague-Dawley 大鼠组与 16 只非禁食同窝大鼠组中的相应肽进行比较，比较大脑中血管活性肠肽 (VIP) 和胆囊收缩素 (CCK) 的浓度和含量，以及肠道中这些肽以及促胰液素和胰高血糖素样免疫反应性 (GLI) 的浓度和含量。研究开始时禁食大鼠的平均体重为 263 +/- 10 g (+/- SEM)，处死前为 177 +/- 7 g，净损失初始体重的 33%； 16 只喂食的老鼠的平均体重从 225 +/- 11 克增加到 284 +/- 12 克，净增 12%。在 5 天的禁食期间，皮质、下丘脑或脑干的重量没有变化。然而，肠道组织的重量下降到喂食动物相应组织重量的一半左右。脑VIP或CCK没有显着变化。肠道中的 VIP 含量没有变化。然而，由于器官重量的减少，其浓度几乎增加了一倍。禁食大鼠肠道内的促胰液素浓度没有显着变化，但器官含量下降到一半左右。 GLI 的肠道含量也下降了一半或更多。禁食动物十二指肠和空肠的甲醇提取物中的 CCK 浓度保持相对恒定，但酸提取物中的 CCK 浓度下降了 40%。这代表 CCK 器官含量大约减少 70%。这些发现可以解释为，在长时间的禁食期间，神经元 CCK 和 VIP 得到很好的保存，但内分泌 CCK、促胰液素和 GLI 由于肠粘膜的损失而显着减少。
The concentrations and contents of vasoactive intestinal peptide (VIP) and cholecystokinin (CCK) in the brain and of these peptides along with secretin and glucagon-like immunoreactivity (GLI) in the gut were compared in a group of 16 5-day fasted adult Sprague-Dawley rats with the corresponding peptides in a group of 16 nonfasted littermates. The mean weight of the fasted rats at the beginning of the study was 263 +/- 10 g (+/- SEM) and was 177 +/- 7 g before killing, for a net loss of 33% of initial body weight; the 16 fed rats increased their mean weight from 225 +/- 11 to 284 +/- 12 g, for a net gain of 12%. During the 5-day fast there was no change in the weight of the cortex, hypothalamus, or brain stem. However, the weight of tissues from the gut decreased to about half the weight of the corresponding tissues in the fed animals. There was no significant change in brain VIP or CCK. VIP content in the gut was unchanged. However, because of the decrease in organ weight, its concentration almost doubled. Secretin concentrations in the gut of fasted rats did not change significantly, but organ contents fell to about half. The gut content of GLI also fell by half or more. The concentrations of CCK in methanol extracts of the duodenum and jejunum remained relatively constant, but those in acid extracts fell by 40% in the fasted animals. This represents an approximately 70% decrease in organ content of CCK. These findings are interpretable as demonstrating that during a prolonged fast neuronal CCK and VIP are well conserved, but endocrine CCK, secretin, and GLI are markedly decreased because of loss of intestinal mucosa.