Combined determination with functional and morphological studies of origin of nerve fibers expressing transient receptor potential vanilloid 1 in the myenteric plexus of the rat jejunum
Combined determination with functional and morphological studies of origin of nerve fibers expressing transient receptor potential vanilloid 1 in the myenteric plexus of the rat jejunum
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DOI：
10.1016/j.autneu.2004.08.005
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发表时间：
2004-11-30
影响因子：
2.7
通讯作者：
Takaki, M
中科院分区：
文献类型：
--
作者：
Kadowaki, M;Kuramoto, H;Takaki, M
本研究的目的是确定辣椒素在离体大鼠小肠的行动和神经纤维表达瞬时受体电位香草素1（TRPV 1：辣椒素受体）在大鼠空肠的功能和免疫组织化学实验相结合的起源。辣椒素（1 μ M）产生延长的松弛反应（对罂粟碱的松弛反应的52.8 ± 15.3%，平均值± S.D.，n=27）的离体空肠中的阿托品和乙啶的存在下。用TRPV 1拮抗剂辣椒平（10 μ M）和钌红（3 μ M）预处理可显著降低辣椒素的舒张反应78%（P
The aim of this study was to determine the action of capsaicin in isolated rat intestine and the origin of nerve fibers expressing transient receptor potential vanilloid 1 (TRPV1: capsaicin receptor) in the rat jejunum by combination of functional and immumohistochenmical experiments. Capsaicin (1 muM) produced a prolonged relaxation response (52.8 +/- 15.3% of the relaxation response to papaverine, mean +/- S.D., n=27) of the isolated jejunum in the presence of atropine and guanethidine. Pretreatment with the TRPV1 antagonist, capsazepine (10 muM) and ruthenium red (3 muM) significantly reduced the relaxation response to capsaicin by 78% (P