ERYTHROPOIETIN MESSENGER-RNA LEVELS IN DEVELOPING MICE AND TRANSFER OF I-125 ERYTHROPOIETIN BY THE PLACENTA
ERYTHROPOIETIN MESSENGER-RNA LEVELS IN DEVELOPING MICE AND TRANSFER OF I-125 ERYTHROPOIETIN BY THE PLACENTA
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DOI：
10.1172/jci113564
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发表时间：
1988-07-01
影响因子：
15.9
通讯作者：
SAWYER, ST
中科院分区：
文献类型：
--
作者：
KOURY, MJ;BONDURANT, MC;SAWYER, ST
测定了正常和贫血小鼠在胎儿和出生后发育过程中促红细胞生成素（EP）mRNA的表达。孕14 d正常胎肝中EP mRNA表达水平较低。到妊娠第19天，在正常或贫血胎肝或正常胎肾中未检测到EP mRNA，但贫血胎肾中EP mRNA水平较低。新生至成年阶段的小鼠通过积累肾脏和肝脏EP mRNA对贫血作出反应。然而，总的肝脏EP mRNA是相当少的肾脏。1-4周龄的幼年动物对贫血反应过度，因为它们产生比成年动物更多的EP mRNA。此外，非贫血青少年很容易测量肾EP mRNA，而成人水平是在检测的下限。由于胎儿EP mRNA水平非常低，因此评价了EP的胎盘转移。当给予妊娠小鼠时，125 I-EP大量转移至胎仔。这些结果表明，在小鼠出生后发育的各个阶段，肾脏都是产生EP的主要器官，成年肾脏也可能通过母体激素的胎盘转移，为胎儿红细胞生成提供EP。
Erythropoietin (EP) mRNA was measured in normal and anemic mice during fetal and postnatal development. Normal fetal livers at 14 d of gestation contained a low level of EP mRNA. By day 19 of gestation, no EP mRNA was detected in normal or anemic fetal livers or normal fetal kidneys, but anemic fetal kidneys had low levels of EP mRNA. Newborn through adult stage mice responded to anemia by accumulating renal and hepatic EP mRNA. However, total liver EP mRNA was considerably less than that of the kidneys. Juvenile animals, 1-4 wk old, were hyperresponsive to anemia in that they produced more EP mRNA than adults. Moreover, nonanemic juveniles had readily measured renal EP mRNA, whereas the adult level was at the lower limit of detection. Because of the very low level of fetal EP mRNA, placental transfer of EP was evaluated. When administered to the pregnant mouse, 125I-EP was transferred in significant amounts to the fetuses. These results indicate that in mice the kidney is the main organ of EP production at all stages of postnatal development and that adult kidney may also play some role in providing EP for fetal erythropoiesis via placental transfer of maternal hormone.