ANTIGEN PRESENTATION BY RAT-BRAIN AND RETINAL ENDOTHELIAL-CELLS
ANTIGEN PRESENTATION BY RAT-BRAIN AND RETINAL ENDOTHELIAL-CELLS
复制标题
DOI：
10.1016/0165-5728(95)00096-k
复制
发表时间：
1995-09-01
影响因子：
3.3
通讯作者：
GREENWOOD, J
中科院分区：
文献类型：
--
作者：
WANG, Y;CALDER, VL;GREENWOOD, J
脑和视网膜内皮细胞（EC）分别形成血脑和血管血视网膜屏障，并被认为在中枢神经系统中介导T细胞反应中发挥作用。在本研究中，体外培养的Lewis大鼠视网膜和脑EC在干扰素γ治疗后能够表达MHC II类I-A分子，但不能表达I-E分子。在其抗原存在下，CD4（+）抗原特异性T细胞能够介导视网膜EC单层的裂解，其程度与脑EC相似。融合的视网膜或脑EC单层对T细胞增殖的支持较差，但亚融合的EC单层以MHC II类（I-A）限制的方式支持增殖（P < 0.001）。将T细胞暴露于融合的视网膜EC单层导致它们对随后由胸腺细胞呈递的抗原反应减弱。相反，亚融合EC预暴露则没有这种影响。这些结果表明，非增殖EC单层能够下调T细胞的反应性，这可能对淋巴细胞穿越中枢神经系统的血液组织屏障具有重要意义。
Brain and retinal endothelial cells (EC) form the blood-brain and vascular blood-retinal barriers, respectively, and are believed to play a role in mediating T cell responses in the central nervous system. In this study, Lewis rat retinal and brain EC grown in vitro were capable of expressing MHC class II I-A but not I-E molecules following treatment with interferon-gamma. In the presence of their antigen, CD4(+) antigen-specific T cells were able to mediate lysis of retinal EC monolayers to a similar extent as brain EC. T cell proliferation was poorly supported by confluent retinal or brain EC monolayers, but subconfluent EC monolayers supported proliferation in a MHC class II (I-A)-restricted manner (P < 0.001). Exposure of T cells to confluent retinal EC monolayers resulted in them becoming less responsive to subsequent antigen presentation by thymocytes. Conversely, pre-exposure with subconfluent EC had no such effect. These results suggest that a non-proliferating EC monolayer is able to downregulate T cell responsiveness which may have important implications during lymphocyte traffic across the blood-tissue barriers of the central nervous system.