The expression of Foxp3 and ROR gamma t in lung tissues from normal smokers and chronic obstructive pulmonary disease patients
The expression of Foxp3 and ROR gamma t in lung tissues from normal smokers and chronic obstructive pulmonary disease patients
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正常吸烟者和慢性阻塞性肺疾病患者肺组织中Foxp3和ROR gamma t的表达。
DOI：
10.1016/j.intimp.2011.06.010
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发表时间：
2011-11-01
影响因子：
5.6
通讯作者：
Bai, Jing
中科院分区：
文献类型：
--
作者：
Chu, Shuyuan;Zhong, Xiaoning;Bai, Jing
Foxp 3和ROR γ t表达细胞参与获得性免疫应答。尚未研究COPD患者和正常吸烟者肺组织中Foxp 3和ROR γ t表达的变化及其在肺气肿中的作用。在本研究中，Foxp 3和ROR γ t采用实时定量聚合酶链反应和蛋白质印迹法进行了评估，Foxp 3，IL-17，IL-23 R和CCR 6的表达和分布，通过免疫组织化学方法进行了测定（10名吸烟者COPD，10名吸烟者和10名非吸烟者肺功能正常）。与吸烟者和非吸烟者相比，COPD患者Foxp 3表达较低，ROR γ t表达较高（所有P值均小于0.001）。Foxp 3/ROR γ t mRNA和蛋白的比值与FEV1%pred呈正相关，与平均肺泡面积呈负相关。COPD患者肺泡壁Foxp 3（+）细胞数较正常吸烟者和非吸烟者减少，而IL-17（+）细胞、IL-23 R（+）细胞和CCR 6（+）细胞数较正常吸烟者和非吸烟者增加（P均<0.001）。IL-17（+）细胞数与CCR 6（+）和IL-23 R（+）细胞数呈正相关。我们的数据显示，COPD患者和正常吸烟者中Foxp 3表达减少和ROR γ t表达增加，这与疾病的加重平行。IL-17（+）细胞相关的细胞因子受体CCR 6和IL-23 R与IL-17（+）细胞增多的机制有关，这将为COPD的免疫治疗提供新的靶点。(C)2011 Elsevier B. V.保留所有权利。
Foxp3- and ROR gamma t-expressing cells are involved in acquired immune responses. The change in Foxp3 and ROR gamma t expression in lung tissue and their role in emphysema has not been studied for COPD patients and normal smokers. In the present study, Foxp3 and ROR gamma t were assessed using real-time quantitative polymerase chain reaction and western blotting, and the expression and distribution of Foxp3, IL-17, IL-23R and CCR6 were measured by immunohistochemistry in peripheral lung tissue (10 smokers with COPD, 10 smokers and 10 nonsmokers with normal lung function). Foxp3 expression was lower and ROR gamma t expression was higher in COPD patients when compared with smokers and nonsmokers (all P values were less than 0.001). The ratios of Foxp3/ROR gamma t mRNA and protein were positively correlated to FEV1%pred and negatively correlated to the mean alveoli area. Foxp3(+) cell numbers were decreased, while the number of IL-17(+) cells, IL-23R(+) cells and CCR6(+) cells were increased in the lung alveolar walls of COPD patients compared with normal smokers and nonsmokers (all P values were less than 0.001). The IL-17(+) cell numbers were positively correlated to both CCR6(+) and IL-23R(+) cells. Our data show a decreased Foxp3 expression and an increased ROR gamma t expression in COPD patients and normal smokers that parallels the aggravation of the disease. The IL-17(+)-cell-related cytokines receptors CCR6 and IL-23R had an association with the mechanism of IL-17(+) cell number increasing, which will provide a new immuno-therapeutic target for COPD. (C) 2011 Elsevier B.V. All rights reserved.