Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges.
Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges.
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DOI：
10.4161/cib.4.4.15250
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发表时间：
2011-07-01
影响因子：
--
通讯作者：
Royle, Stephen J
中科院分区：
文献类型：
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作者：
Cheeseman, Liam P;Booth, Daniel G;Royle, Stephen J
在有丝分裂过程中，染色体的精确分离是通过纺锤体的动粒纤维（K-纤维）实现的。这些纤维是由非运动桥连接的微管（MT）束。我们最近确定了一个TACC 3/ch-TOG/网格蛋白复合物，构成了K-纤维中最短的MT间桥。TACC 3将复合物锚定到MT，这依赖于Aurora A激酶的磷酸化。在此，我们发现使用MLN 8237抑制Aurora A激酶导致（1）纺锤体网格蛋白和TACC 3的丢失，（2）K纤维的不稳定和（3）MT间桥的丢失。这些结果与耗尽网格蛋白或TACC 3的细胞中的结果相似;表明TACC 3/ch-TOG/网格蛋白桥是由该激酶调节的主要类型的桥。
Accurate chromosome segregation during mitosis is achieved by the kinetochore fibers (K-fibers) of the spindle apparatus. These fibers are bundles of microtubules (MTs) connected by non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes the shortest class of inter-MT bridge in K-fibers. TACC3 anchors the complex to MTs and this is dependent on phosphorylation by Aurora A kinase. Here we show that inhibition of Aurora A kinase using MLN8237 results in (1) loss of clathrin and TACC3 from spindles, (2) destabilization of K-fibers and (3) loss of inter-MT bridges. These results are similar to those in cells depleted of clathrin or TACC3; suggesting that TACC3/ch-TOG/clathrin bridges are the major class of bridge that is regulated by this kinase.