Light wavelength and intensity effects on larval settlement in the Pacific oyster Magallana gigas
Light wavelength and intensity effects on larval settlement in the Pacific oyster Magallana gigas
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光波长和强度对太平洋牡蛎 Magallana gigas 幼虫沉降的影响
DOI：
10.1007/s10750-021-04550-9
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发表时间：
2021
期刊：
影响因子：
2.6
通讯作者：
Satuito Cyril Glenn
中科院分区：
文献类型：
--
作者：
Kim Hee-Jin;Suematsu Yumi;Kaneda Hayato;Satuito Cyril Glenn
牡蛎幼虫最初在定居阶段有一个眼斑（pediveligers）。本文研究了太平洋牡蛎Magallana gigaslarvae的眼斑与光的波长依赖性的吸收，其功能的细节定居。眼点有效吸收500-650 nm的光波长。基于眼斑吸光度，用发光二极管（LED）使pediveliger经受不同的光条件：近紫外（NUV）、蓝色、白色、绿色、红色和近红外（NIR），设置为3种强度（5、15、25 W/m2，NIR除外，其为25、50、100 lux）。将以每个强度调制的LED连续照射2天，并且每天计数在壳片基底上沉降的幼虫的数量。15、25 W/m2的NUV和5、15 W/m2的白色和绿色光对幼虫的附着有干扰作用，而25 W/m2的红光和100 lux的NIR则表现出较强的附着诱导活性。红色和NIR进一步用陶瓷纸基材;惰性基材测试。这些灯保持了它们的性能，在黑暗中的沉降率高于对照。研究结果有助于进一步认识眼点在牡蛎幼体定居中的作用，并为建立光污染水平评价标准，保护底栖生态系统提供参考。
Oyster larvae initially have an eyespot on settlement stage (pediveligers). This study investigated the eyespot with light wavelength-dependent absorbance, and its functional details for settlement in the Pacific oysterMagallana gigaslarvae. Eyespot efficiently absorbed 500–650 nm of light wavelength. Based on the eyespot absorbance, pediveligers were subjected to different light conditions with light-emitting diodes (LEDs): near ultraviolet (NUV), blue, white, green, red, and near infrared (NIR), set at 3 intensities (5, 15, 25 W/m2, except for NIR, which was at 25, 50, 100 lux). LEDs modulated at each intensity were continuously irradiated for 2 days, and the number of settled larvae on a substrate of shell chips was counted daily. NUV at 15, 25 W/m2, and white and green light at 5, 15 W/m2disrupted larval settlement, whereas red at 25 W/m2and NIR at 100 lux showed strong settlement-inducing activities. Red and NIR were further tested with ceramic paper substrates; inert substrates. These lights maintained their performance, with settlement rates higher than controls in darkness. Our findings contribute to the further understanding of the function of eyespot in the settlement of oyster larvae, and to establish criteria to evaluate light pollution levels for protecting benthic ecosystem.