Applying fluorescence lifetime imaging microscopy to evaluate the efficacy of anticancer drugs
Applying fluorescence lifetime imaging microscopy to evaluate the efficacy of anticancer drugs
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DOI：
10.1088/2050-6120/3/2/025006
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发表时间：
2015-06-01
影响因子：
3.2
通讯作者：
Imasaka, Totaro
中科院分区：
文献类型：
--
作者：
Kawanabe, Satoshi;Araki, Yoshie;Imasaka, Totaro
荧光寿命成像显微镜用于评价抗癌药物的疗效。在用抗肿瘤抗生素如阿霉素或表阿霉素处理后，检测到SYTO 13染料染色的凋亡癌细胞中细胞核的荧光寿命降低。证实了荧光寿命的变化早于细胞中的形态变化发生。我们发现，在细胞核的荧光寿命与表阿霉素处理的细胞比，更迅速地减少。这意味着表阿霉素在治疗癌细胞方面比阿霉素更有效。然而，当细胞用环磷酰胺处理时，未显示荧光寿命的变化。荧光寿命的减少与涉及半胱天冬酶激活和染色质凝聚的过程有关。因此，这项技术将提供有关凋亡细胞的有用信息，特别是在早期阶段。
Fluorescence lifetime imaging microscopy was applied to evaluate the efficacy of anticancer drugs. A decrease in the fluorescence lifetime of the nucleus in apoptotic cancer cells stained by SYTO 13 dye was detected after treatment with antitumor antibiotics such as doxorubicin or epirubicin. It was confirmed that the change in fluorescence lifetime occurred earlier than morphological changes in the cells. We found that the fluorescence lifetime of the nucleus in the cells treated with epirubicin decreased more rapidly than that of the cells treated with doxorubicin. This implies that epirubicin was more efficacious than doxorubicin in the treatment of cancer cells. The change in fluorescence lifetime was, however, not indicated when the cells were treated with cyclophosphamide. The decrease in fluorescence lifetime was associated with the processes involving caspase activation and chromatin condensation. Therefore, this technique would provide useful information about apoptotic cells, particularly in the early stages.