Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels
Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels
复制标题
离子辅助制备中性蛋白交联海藻酸钠纳米凝胶
DOI：
10.1016/j.carbpol.2018.01.035
复制
发表时间：
2018-04-15
影响因子：
11.2
通讯作者：
Huang, Yubin
中科院分区：
文献类型：
--
作者：
Wei, Xing;Xiong, Hejian;Huang, Yubin
蛋白质交联纳米凝胶结合了纳米凝胶的优点和蛋白质的特定生物活性，已成为令人着迷的蛋白质递送系统。然而，蛋白质中反应基团的脆性和低密度，特别是中性蛋白质的低电密度严重限制了蛋白质交联纳米凝胶的制备而不影响其生物活性。在这里，我们展示了一种简便的离子辅助方法来制造中性蛋白质交联纳米凝胶。成功合成了具有醛基、低粘度的氧化海藻酸钠（OSA），它可以通过添加和去除二价阳离子可逆地形成纳米凝胶。随后，以血红蛋白和肌红蛋白为代表的中性蛋白，在二价阳离子的辅助下制备稳定的蛋白交联OSA纳米凝胶，然后在OSA和蛋白之间原位形成希夫碱。温和的制备条件保证了所得蛋白质交联纳米凝胶中蛋白质的结构完整性和生物活性。这种离子辅助方法有望为制造多功能功能生物杂化纳米凝胶系统带来新的机会。
Protein crosslinked nanogels which combine the merits of nanogels and the specific biological activity from protein have emerged as fascinating protein delivery systems. However, the fragility and low density of reactive group in proteins, especially low electric density of neutral proteins seriously limited the fabrication of protein crosslinked nanogels without affecting their bioactivities. Here, we demonstrated a facile ion-assisted method to fabricate neutral protein crosslinked nanogels. Oxidized sodium alginate (OSA) with aldehyde groups and low viscosity was successfully synthesized, which could reversibly form nanogels via addition and removal of divalent cation. Subsequently, hemoglobin and myoglobin were used as representative neutral proteins to fabricate stable protein crosslinked OSA nanogels under the assist of divalent cation followed by in situ Schiff base formation between OSA and proteins. The mild fabrication condition guaranteed the structural integrity and bioactivity of proteins in the obtained protein crosslinked nanogels. This ion-assisted method was expected to bring a new opportunity for fabricating versatile functional biohybrid nanogels systems.