Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.
Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.
复制标题
角蛋白丝的驱动蛋白依赖性运输：中间丝运输的统一机制。
DOI：
10.1096/fj.201800604r
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Gelfand,VladimirI
中科院分区：
文献类型：
--
作者：
Robert,Amélie;Tian,Peirun;Adam,StephenA;Kittisopikul,Mark;Jaqaman,Khuloud;Goldman,RobertD;Gelfand,VladimirI
Keratin intermediate filaments (IFs) are the major cytoskeletal component in epithelial cells. The dynamics of keratin IFs have been described to depend mostly on the actin cytoskeleton, but the rapid transport of fully polymerized keratin filaments has not been reported. In this work, we used a combination of photoconversion experiments and clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats–associated protein 9 genome editing to study the role of microtubules and microtubule motors in keratin filament transport. We found that long keratin filaments, like other types of IFs, are transported along microtubules by kinesin-1. Our data revealed that keratin and vimentin are nonconventional kinesin-1 cargoes because their transport did not require kinesin light chains, which are a typical adapter for kinesin-dependent cargo transport. Furthermore, we found that the same domain of the kinesin heavy chain tail is involved in keratin and vimentin IF transport, strongly suggesting that multiple types of IFs move along microtubules using an identical mechanism.—Robert, A., Tian, P., Adam, S. A., Kittisopikul, M., Jaqaman, K., Goldman, R. D., Gelfand, V. I. Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.
