OVGP1 is an oviductal fluid factor essential particularly for early embryonic development in golden hamsters
OVGP1 is an oviductal fluid factor essential particularly for early embryonic development in golden hamsters
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OVGP1 是一种输卵管液因子，对于金黄仓鼠的早期胚胎发育尤其重要
DOI：
10.1101/2022.10.25.513711
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Araki Yoshihiko
中科院分区：
文献类型：
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作者：
Yamatoya Kenji;Kurosawa Masaru;Hirose Michiko;Miura Yoshiki;Taka Hikari;Nakano Tomoyuki;Hasegawa Akiko;Kagami Kyosuke;Yoshitake Hiroshi;Goto Kaoru;Ueno Takashi;Fujiwara Hiroshi;Shinkai Yoichi;Kan Frederick W. K.;Ogura Atsuo;Araki Yoshihiko
哺乳动物输卵管腔是一个专门的腔室，它提供了一个严格调节受精和早期胚胎发生的环境，配子/合子的调节机制仍然是未知的。本研究利用OVGP 1基因敲除（KO）仓鼠研究了输卵管对早期胚胎发育的调控作用。实验结果表明：1）Ovgp 1-KO雌性仓鼠完全不能产仔; 2）KO动物输卵管中有时能识别出受精卵，但形态学上有异常; 3）KO雌性仓鼠受精卵的数量明显较少; 4）即使发生受精卵，胚胎发育也异常，最终成为胚胎致死;和5）转移到野生型雌性的Ovgp 1-KO雌性产生KO卵衍生的窝仔，但反向实验不产生。这些结果表明，OVGP 1介导的生理事件是至关重要的早期胚胎发育体内。该动物模型表明，受精卵的命运不仅由基因决定，而且周围的输卵管微环境在正常胚胎发育中起着关键作用。概要声明输卵管体液因子（OVGP 1）缺乏导致金黄地鼠雌性不育。OVGP 1的存在或缺失对早期胚胎的体内发育具有重要的生理作用。
The mammalian oviductal lumen is a specialized chamber that provides an environment that strictly regulates fertilization an early embryogenesis, the regulatory mechanisms to gametes/zygote are still largely unknown. In this report, we studied the oviductal regulation of early embryonic development usingOvgp1(a gene encoding an oviductal humoral factor, OVGP1)-knockout (KO) hamsters. The experimental results revealed the following: 1) FemaleOvgp1-KO hamsters fail to produce any litters at all; 2) In the oviducts from KO animal, fertilized eggs are sometimes identified, but their morphology shows abnormal features; 3) The number of implantations in the KO females is evidently low; 4) Even if implantations occur, the embryos develop abnormally and eventually become embryonic lethal; and 5)Ovgp1-KO females transferred to wild-type females produce KO egg-derived litters, but the reverse experiment does not. These results suggest that OVGP1-mediated physiological events are crucial for early embryonic developmentin vivo. This animal model shows that the fate of the fertilized egg is not only genetically determined, but that the surrounding oviductal microenvironment plays a pivotal role in normal embryonic development.Summary statementDeficiency an oviductal humoral factor (OVGP1) caused female infertility in the golden hamsters. The presence or absence of OVGP1 has significant physiological effects on early embryonic developmentin vivo.