H2S improves salt-stress recovery via organic acid turn-over in apple seedlings
H2S improves salt-stress recovery via organic acid turn-over in apple seedlings
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H 2 S 通过有机酸转化改善苹果幼苗的盐胁迫恢复
DOI：
10.1111/pce.14410
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发表时间：
2022-08-16
影响因子：
7.3
通讯作者：
Ma, Fangfang
中科院分区：
文献类型：
--
作者：
Du, Minghui;Zhang, Peng;Ma, Fangfang
硫化氢（H2S）在胁迫生物学中的信号作用被广泛报道，但尚未充分确定，以敦促其在农艺实践中的使用。我们缺乏对H2S信号传导中代谢重布线的定量理解，使得难以在生化水平上阐明其在胁迫耐受性中的功能。这里，湖北海棠。变种用盐胁迫2周后，再用4种不同浓度的NaHS处理平邑甜茶幼苗。通过表型分析、C-13瞬时标记、靶向代谢和转录组学分析等研究，我们首次在木本果树幼苗中发现，H2S通过糖酵解和三羧酸循环固定碳，抑制碳水化合物的无效积累，维持有效的CO2同化，保持淀粉代谢平衡，产生足够的H2 O2，通过H2 O2诱导的阴离子通道（铝激活的苹果酸转运蛋白）维持苹果酸/γ-氨基丁酸稳态，并最终改善盐胁迫恢复。我们的研究结果系统地证明了中心碳代谢在H2S信号转导中的重要作用，并阐明了H2S在苹果幼苗中的作用模式。我们得出结论，H2S信号与中央碳代谢自下而上的方式恢复植物生长盐胁迫后。
Signalling roles of hydrogen sulphide (H2S) in stress biology are widely reported but not sufficiently established to urge its use in agronomic practice. Our lack of quantitative understanding of the metabolic rewiring in H2S signalling makes it difficult to elucidate its functions in stress tolerance on the biochemical level. Here, Malus hupehensis Rehd. var. pingyiensis seedlings were first treated with salt stress for 2 weeks and then treated with four different concentrations of NaHS. Through vigorous investigations, including phenotypic analysis, C-13 transient labelling and targeted metabolic and transcriptomic analysis, for the first time in the seedlings of a woody fruit crop, we found out that H2S recycles fixed carbons through glycolysis and tricarboxylic acid cycle to inhibit the futile accumulation of carbohydrates, to maintain an efficient CO2 assimilation, to keep a balanced starch metabolism, to produce sufficient H2O2, to maintain malate/gamma-aminobutyric acid homeostasis via an H2O2-induced anion channel (aluminium-activated malate transporter) and eventually to improve salt-stress recovery. Our results systematically demonstrate the vital roles of central carbon metabolism in H2S signalling and clarify the mode of action of H2S in apple seedlings. We conclude that H2S signalling interacts with central carbon metabolism in a bottom-up manner to recover plant growth after salt stress.