A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES
A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES
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DOI：
10.1073/pnas.85.18.6622
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发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
WU, JR
中科院分区：
文献类型：
--
作者：
MOYZIS, RK;BUCKINGHAM, JM;WU, JR
从人重组重复DNA文库中分离到一个高度保守的重复DNA序列（TTAGGG）n。与通过流式细胞术分选的染色体的定量杂交表明，在每个人染色体上存在相当数量的该序列。荧光原位杂交和BAL-31核酸酶消化实验都揭示了该序列在所有人类染色体端粒的主要簇。该DNA序列的进化保守性、其在各种高等真核生物中的末端染色体位置（无论染色体数目或染色体长度）以及其与从低等真核生物分离的功能性端粒的相似性表明该序列是功能性人类端粒。
A highly conserved repetitive DNA sequence, (TTAGGG)n, has been isolated from a human recombinant repetitive DNA library. Quantitative hybridization to chromosomes sorted by flow cytometry indicates that comparable amounts of this sequence are present on each human chromosome. Both fluorescent in situ hybridization and BAL-31 nuclease digestion experiments reveal major clusters of this sequence at the telomeres of all human chromosomes. The evolutionary conservation of this DNA sequence, its terminal chromosomal location in a variety of higher eukaryotes (regardless of chromosome number or chromosome length), and its similarity to functional telomeres isolated from lower eukaryotes suggest that this sequence is a functional human telomere.