INDUCTION OF NORMAL CARDIOVASCULAR DEVELOPMENT IN THE VITAMIN-A-DEPRIVED QUAIL EMBRYO BY NATURAL RETINOIDS
INDUCTION OF NORMAL CARDIOVASCULAR DEVELOPMENT IN THE VITAMIN-A-DEPRIVED QUAIL EMBRYO BY NATURAL RETINOIDS
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DOI：
10.1006/dbio.1993.1318
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发表时间：
1993-12-01
影响因子：
2.7
通讯作者：
ZILE, MH
中科院分区：
文献类型：
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作者：
DERSCH, H;ZILE, MH
在维生素A缺乏的日本鹌鹑胚胎中，研究了各种天然类维生素A的生物活性和正常心血管发育所需维生素A活性的时间“窗口”。在孵育开始时给予1 μg视黄醇，97%的胚胎心血管发育正常;维甲酸在该剂量水平下具有毒性。在孵育开始时，用0.1 μg全反式视黄醇或13-顺式维甲酸处理胚胎，分别导致47%和12%的胚胎心血管发育正常;在其他时间点给予这些类维生素A，降低了心血管发育正常的胚胎百分比。二脱氢视黄醇（0.1 μg）和9-顺式视黄酸（0.1 μg）在所有检查时间点均无活性; 9-顺式视黄酸未增强全反式视黄酸的生物活性。全反式维甲酸，0.1 μg，在22-28小时的孵育期间给药，诱导20-34%的胚胎正常心血管发育;生物活性在24小时给药时最佳。所有试验的维甲酸在孵育36小时或更晚时给药时，在建立正常心血管发育方面无活性。研究表明，全反式维甲酸是鸟类胚胎正常心血管发育所需的维生素A的生物活性形式。在鹌鹑胚胎发生的前22-28小时内有一个关键的时间点，此时全反式维甲酸启动导致正常心血管发育的事件。
The biological activity of various natural retinoids and the time "window" when vitamin A activity is required for normal cardiovascular development were examined in vitamin A-deprived Japanese quail embryos. The administration of 1 μg of retinol at the beginning of incubation resuited in normal cardiovascular development in 97% of embryos; retinoic acid was toxic at this dose level. Treatment of embryos with 0.1 μg of all-trans-retinol or 13-cis-retinoic acid at the beginning of incubation resulted in normal cardiovascular development in 47 and 12% of embryos, respectively; administration of these retinoids at other time points attenuated the percentage of embryos with normal cardiovascular development. Didehydroretinol, 0.1 μg, and 9-cis-retinoic acid, 0.1 μg, were inactive at all time points examined; 9-cis-retinoic acid did not enhance the biological activity of all-trans-retinoic acid. All-trans-retinoic acid, 0.1 μg, administered during 22-28 hr of incubation induced normal cardiovascular development in 20-34% of embryos; biological activity was optimal when it was administered at 24 hr. All retinoids tested were inactive in establishing normal cardiovascular development when administered at 36 hr of incubation or later. The studies suggest that all-trans-retinoic acid is the biologically active form of vitamin A required for normal cardiovascular development in the avian embryo. There is a critical time point within the first 22-28 hr of quail embryogenesis when all-trans-retinoic acid initiates events that lead to normal cardiovascular development.