Electroconvulsive shocks increase the concentration of neocortical and hippocampal neuropeptide Y (NPY)-like immunoreactivity in the rat.
Electroconvulsive shocks increase the concentration of neocortical and hippocampal neuropeptide Y (NPY)-like immunoreactivity in the rat.
复制标题
电休克会增加大鼠新皮质和海马神经肽 Y (NPY) 样免疫反应性的浓度。
DOI：
10.1016/0006-8993(90)90523-e
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Ekman,R
中科院分区：
文献类型：
--
作者：
Wahlestedt,C;Blendy,JA;Kellar,KJ;Heilig,M;Widerlöv,E;Ekman,R
对人类和大鼠的研究表明，神经肽Y （NPY）与重度抑郁和焦虑有关。因此，我们进行了本研究，以阐明反复（13或14天）电休克（ECS）治疗大鼠对脑各区域、肾上腺和血浆中npy样免疫反应性（-LI）浓度的影响。此外，我们还研究了ECS在3个脑区对125i - npy结合的影响。将ECS的效果与3种对照治疗的效果进行比较：一组在此期间完全不处理，一组与ECS组一样处理但不接受电击，一组接受低于诱发惊厥阈值的电击。后一组出现的行为迹象让人想起不可避免的由电击引起的“习得性无助”综合症（一种被提出的抑郁症动物模型）。我们发现，与3个对照组相比，ecs组在额叶和顶叶皮层以及海马中的NPY-LI浓度大约增加了一倍。纹状体、下丘脑、脑桥、嗅球、小脑、血浆和肾上腺均未检测到NPY-LI的变化。尽管NPY-LI浓度变化明显，但4组大鼠的额顶皮质和海马中125i - npy的结合特征相似。最后，我们证实了之前的观察结果，即ECS增加了[3H]prazosin在皮层的结合。总之，ECS治疗增加了新皮质和海马的NPY-LI浓度，而125i - npy结合不受影响。亚惊厥休克没有效果。
Studies on humans and rats have suggested that neuropeptide Y (NPY) is involved in major depression and anxiety. Therefore, we conducted the present study in order to elucidate the effect of repeated (13 or 14 days) treatment of rats with electroconvulsive shocks (ECS) on the concentration of NPY-like immunoreactivity (-LI) in various brain regions, adrenals and plasma. In addition, the effect of ECS on125I-NPY binding was studied in 3 brain regions. The effects of ECS were compared to effects of 3 control treatments: one group not being handled at all during the time period, one group handled like the ECS-group but not receiving shocks, and one group receiving shocks below the threshold for induction of convulsions. The latter group developed behavioural signs reminiscent of the inescapable shock-induced ‘learned helplessness’ syndrome (a proposed animal model of depression). We found that the concentration of NPY-LI in the frontal and parietal cortex and in the hippocampus were approximately doubled in the ECS-group as compared to the 3 control groups. No changes in NPY-LI were detected in the striatum, hypothalamus, pons, olfactory bulbs or cerebellum, nor in plasma or adrenals. In spite of the marked changes NPY-LI concentration, the binding characteristics of125I-NPY in the frontal and parietal cortex and in the hippocampus were similar in all 4 groups of rats. Finally, we confirmed the previous observationnthat ECS increase [3H]prazosin binding in cortex. In conclusion, ECS treatment increases neocortical and hippocampal NPY-LI concentrations, while leaving125I-NPY binding unaffected. Subconvulsive shocks were without effect.