Glycoprofiling as a novel tool in serological assays of systemic sclerosis: a comparative study with three bioanalytical methods.
Glycoprofiling as a novel tool in serological assays of systemic sclerosis: a comparative study with three bioanalytical methods.
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DOI：
10.1016/j.aca.2014.10.029
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发表时间：
2015-01-01
影响因子：
6.2
通讯作者：
Tkac J
中科院分区：
文献类型：
--
作者：
Klukova L;Bertok T;Petrikova M;Sediva A;Mislovicova D;Katrlik J;Vikartovska A;Filip J;Kasak P;Andicsová-Eckstein A;Mosnáček J;Lukáč J;Rovenský J;Imrich R;Tkac J
系统性硬化症（SSc）是一种严重影响患者生活质量的自身免疫性疾病。该疾病的异质性也意味着该疾病的生物标志物的鉴定和后续验证相当具有挑战性。目前还没有一种完全有效的用于诊断、预后、疾病活动性和治疗反应评估的单一生物标志物。本研究的主要目的是利用唾液酸识别凝集素黑Sambucus nigra凝集素（SNA）对糖谱血清样本进行糖谱分析，应用一种基于免疫分析的替代检测方案。据我们所知，这是第一个描述血清SSc样品直接基于凝集素的糖谱分析的研究。本文从生物分析的角度比较了基于凝集素应用的三种不同的血清糖谱分析方法，包括传统的基于elisa的凝集素方法（ELLA）、新型荧光凝集素微阵列和超灵敏阻抗凝集素生物传感器。当将健康人的血清与SSc患者的血清进行比较时，所有三种生物分析方法获得的结果一致显示糖蛋白上唾液酸的水平存在差异。因此，分析人血清中的唾液酸含量可能对未来的SSc检测具有诊断价值，但需要进一步的工作来提高检测的选择性，例如通过对富含抗体的部分人血清进行糖谱分析来进行个体诊断。
Systemic sclerosis (SSc) is an autoimmune disease seriously affecting patient´s quality of life. The heterogeneity of the disease also means that identification and subsequent validation of biomarkers of the disease is quite challenging. A fully validated single biomarker for diagnosis, prognosis, disease activity and assessment of response to therapy is not yet available. The main aim of this study was to apply an alternative assay protocol to the immunoassay-based analysis of this disease by employment of sialic acid recognizing lectin Sambucus nigra agglutinin (SNA) to glycoprofile serum samples. To our best knowledge this is the first study describing direct lectin-based glycoprofiling of serum SSc samples. Three different analytical methods for glycoprofiling of serum samples relying on application of lectins are compared here from a bioanalytical point of view including traditional ELISA-like lectin-based method (ELLA), novel fluorescent lectin microarrays and ultrasensitive impedimetric lectin biosensors. Results obtained by all three bioanalytical methods consistently showed differences in the level of sialic acid present on glycoproteins, when serum from healthy people was compared to the one from patients having SSc. Thus, analysis of sialic acid content in human serum could be of a diagnostic value for future detection of SSc, but further work is needed to enhance selectivity of assays for example by glycoprofiling of a fraction of human serum enriched in antibodies for individual diagnostics.