Metabolism of platelet-activating factor in isolated perfused rat lung.
Metabolism of platelet-activating factor in isolated perfused rat lung.
复制标题
离体灌注大鼠肺中血小板激活因子的代谢。
DOI：
10.1172/jci113028
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Murphy,RC
中科院分区：
文献类型：
--
作者：
Haroldsen,PE;Voelkel,NF;Henson,JE;Henson,PM;Murphy,RC
血小板活化因子（PAF）进入肺的气道系统的管理是众所周知的，造成深远的影响，但很少有人知道这种活性脂质介质的代谢。~ 3 H-标记的PAF经离体大鼠肺气道给药后迅速而广泛地代谢。组织保留了96%的给药放射性标记，而灌注液中含有4%。组织脂质的特征表明，代谢为溶血PAF（3.3%），磷脂酰胆碱（82.3%），中性脂质（1.7%）和完整的PAF（10.2%）。通过质谱技术对组织磷脂酰胆碱的分析显示PAF代谢为1-0-十六烷基-2-花生四烯酸-GPC，占给药放射性标记十六烷基-PAF的20-23%。这些发现支持PAF和花生四烯酸代谢之间的关系存在于完整器官水平的假设。放射性标记的PAF代谢物在肺组织中的细胞分布的放射自显影分析表明标记了两种细胞类型，肺泡II型细胞和无纤毛细支气管上皮细胞（Clara细胞）。
The administration of platelet-activating factor (PAF) into the airway system of the lung is known to cause profound effects, yet little is known about the metabolism of this active lipid mediator. 3H-Labeled PAF administered into the airway of isolated rat lungs was rapidly and extensively metabolized. The tissue retained 96% of the administered radiolabel while the perfusate contained 4%. Characterization of the tissue retained lipid indicated metabolism into lyso-PAF (3.3%), phosphatidylcholine (82.3%), neutral lipid (1.7%) and intact PAF (10.2%). Analysis of tissue phosphatidylcholine by mass spectrometric techniques revealed metabolism of PAF to 1-0-hexadecyl-2-arachidonoyl-GPC, which represented 20-23% of the administered radiolabeled hexadecyl-PAF. These findings support the hypothesis that a relationship between PAF and arachidonate metabolism exists at the intact organ level. Autoradiographic analysis of the cellular distribution of the radiolabeled PAF metabolites in the lung tissue indicated labeling of two cell types, the alveolar type II cell and the nonciliated bronchiolar epithelial cell (Clara cell).Images