Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length
Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length
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DOI：
10.1007/s001220100661
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发表时间：
2001-11-01
影响因子：
5.4
通讯作者：
Kato, A
中科院分区：
文献类型：
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作者：
Saito, K;Miura, K;Kato, A
Norin-PL8是水稻（Oryza sativa L.）的一个耐寒品种，它是由耐寒的爪哇品种Silewah染色体片段渐渗而成的。我们之前在3号和4号染色体的渗入中检测到了Norin-PL8的耐寒性数量性状位点（qtl）。我们提供了4号染色体上qtl的精细定位，以及qtl与花药长度之间的关联，据报道花药长度是耐寒性的主要组成部分。利用来自高级回交后代的分离群体进行的区间定位表明，耐寒QTL可能位于渗入的中心到近端。为了精细定位，我们从分离群体中的重组体中开发了一组近等基因系（NILs）。两个基因的耐寒性比较表明，基因渗入的近端或中心对耐寒性都是必需的。从这些结果中，我们得出结论，至少有两个与耐冷性有关的qtl，暂定为Ctb-1和Ctb-2。在4号染色体的渗进中。Ctb-1和Ctb-2之间的地图距离估计为4.7-17.2 cM。为了研究这些qtl耐寒性的机制，我们比较了NILs的花药长度。结果表明，Ctb-1和Ctb-2均与花药长度有关。
Norin-PL8 is a cold-tolerant variety of rice (Oryza sativa L.) that was developed by introgressing chromosomal segments from a cold-tolerant javanica variety, Silewah. We previously detected quantitative trait loci (QTLs) for cold tolerance of Norin-PL8 in the introgressions on chromosomes 3 and 4. We provide fine mapping of the QTLs on chromosome 4 and the association between the QTLs and anther length, which has been reported to be a major component of cold tolerance. Interval mapping using a segregating population derived from an advanced backcross progeny indicated that a QTL for cold tolerance is probably located from the center to the proximal end of the introgression. For fine mapping, we developed a set of near-isogenic lines (NILs) from recombinants in the segregating population. Comparison of cold tolerance between the NILs indicated that either the proximal end or the center of the introgression is necessary for cold tolerance. From these results, we concluded that there are at least two QTLs for cold tolerance, tentatively designated as Ctb-1 and Ctb-2. in the introgression on chromosome 4. The map distance between Ctb-1 and Ctb-2 is estimated to be 4.7-17.2 cM. In order to investigate the mechanism underlying cold tolerance by the QTLs, we compared anther lengths of the NILs. The results indicate that both Ctb-1 and Ctb-2 are associated with anther length.