Using CRISPRs as a metagenomic tool to identify microbial hosts of a diffuse flow hydrothermal vent viral assemblage
Using CRISPRs as a metagenomic tool to identify microbial hosts of a diffuse flow hydrothermal vent viral assemblage
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DOI：
10.1111/j.1574-6941.2011.01090.x
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发表时间：
2011-07-01
影响因子：
4.2
通讯作者：
Baross, John A.
中科院分区：
文献类型：
--
作者：
Anderson, Rika E.;Brazelton, William J.;Baross, John A.
病毒的宏基因组分析揭示了病毒圈的广泛多样性，但确定病毒组合的特定宿主仍然是一个挑战。为了解决这一问题，我们分析了东北太平洋热液喷口的病毒宏基因组，并使用了一个综合的间隔序列数据库，这些间隔序列来自集群规则间隔短回文重复序列（CRISPR）推定免疫系统。CRISPR间隔序列与海洋喷口病毒组的匹配表明，在这种喷口环境中存在感染不同分类群体宿主的病毒。比较病毒组分析表明，来自喷口分离物和嗜热菌的CRISPR间隔物与喷口病毒组的匹配率高于其他海洋或陆地温泉病毒组。然而，中温性宿主对间隔物的高命中率，加上中等高的模拟α多样性，表明海洋喷口病毒组由病毒组成，这些病毒有可能感染细菌和古细菌领域中多种热状态的不同分类群。
Metagenomic analyses of viruses have revealed widespread diversity in the viriosphere, but it remains a challenge to identify specific hosts for a viral assemblage. To address this problem, we analyze the viral metagenome of a northeast Pacific hydrothermal vent with a comprehensive database of spacers derived from the clustered regularly interspaced short palindromic repeat (CRISPR) putative immune system. CRISPR spacer matches to the marine vent virome suggest that viruses infecting hosts from diverse taxonomic groups are present in this vent environment. Comparative virome analyses show that CRISPR spacers from vent isolates and from thermophiles in general have a higher percentage of matches to the vent virome than to other marine or terrestrial hot spring viromes. However, a high percentage of hits to spacers from mesophilic hosts, combined with a moderately high modeled alpha diversity, suggest that the marine vent virome is comprised of viruses that have the potential to infect diverse taxonomic groups of multiple thermal regimes in both the bacterial and the archaeal domains.