Complete Genome Sequences of Rat and Mouse Segmented Filamentous Bacteria, a Potent Inducer of Th17 Cell Differentiation
Complete Genome Sequences of Rat and Mouse Segmented Filamentous Bacteria, a Potent Inducer of Th17 Cell Differentiation
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DOI：
10.1016/j.chom.2011.08.007
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发表时间：
2011-09-15
影响因子：
30.3
通讯作者：
Hattori, Masahira
中科院分区：
文献类型：
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作者：
Prakash, Tulika;Oshima, Kenshiro;Hattori, Masahira
分段丝状细菌 (SFB) 是一种不可培养的共生菌，栖息在各种脊椎动物的肠道中，并已被证明可以在小鼠中诱导 Th17 细胞。我们展示了从 SFB 单一定植宿主中分离出的大鼠和小鼠 SFB 的完整基因组序列。大鼠和小鼠 SFB 基因组各自包含一条 1.52 和 1.59 Mb 的圆形染色体，分别编码 1346 和 1420 个蛋白质编码基因。两个基因组之间的总体核苷酸同一性为 86%，估计替换率与自由生活的大肠杆菌相似。 SFB基因组编码厌氧发酵、孢子和鞭毛形成的典型基因，但缺乏大多数氨基酸生物合成酶，让人想起致病性梭状芽胞杆菌，对宿主表现出很大的依赖性。然而，SFB 缺乏大部分梭菌毒力相关基因。与梭菌基因组的比较分析表明了宿主反应和肠道特定适应的可能机制。
Segmented filamentous bacteria (SFB) are noncultivable commensals inhabiting the gut of various vertebrate species and have been shown to induce Th17 cells in mice. We present the complete genome sequences of both rat and mouse SFB isolated from SFB-monocolonized hosts. The rat and mouse SFB genomes each harbor a single circular chromosome of 1.52 and 1.59 Mb encoding 1346 and 1420 protein-coding genes, respectively. The overall nucleotide identity between the two genomes is 86%, and the substitution rate was estimated to be similar to that of the free-living E. coli. SFB genomes encode typical genes for anaerobic fermentation and spore and flagella formation, but lack most of the amino acid biosynthesis enzymes, reminiscent of pathogenic Clostridia, exhibiting large dependency on the host. However, SFB lack most of the clostridial virulence-related genes. Comparative analysis with clostridial genomes suggested possible mechanisms for host responses and specific adaptations in the intestine.