Phosphorylation of Intrinsically Disordered Starmaker Protein Increases Its Ability To Control the Formation of Calcium Carbonate Crystals
Phosphorylation of Intrinsically Disordered Starmaker Protein Increases Its Ability To Control the Formation of Calcium Carbonate Crystals
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DOI：
10.1021/cg200905f
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发表时间：
2012-01
影响因子：
3.8
通讯作者：
M. Wojtas;M. Wołcyrz;A. Ożyhar;P. Dobryszycki
中科院分区：
文献类型：
--
作者：
M. Wojtas;M. Wołcyrz;A. Ożyhar;P. Dobryszycki
斑马鱼的内耳含有称为耳石的生物矿物，由碳酸钙晶体和蛋白质有机基质组成。先前已经提出，Starmaker（Stm）是一种内在无序的蛋白质，作为耳石的组成部分，控制晶体的大小，形状和多晶型。在这项研究中，在体外碳酸钙结晶系统的建立，以检查的作用，Stm和广泛磷酸化的Stm（StmP）在晶体的形成。SEM和X-射线衍射分析表明，方解石晶体的尺寸生长在Stm的存在下，已被磷酸化的CK 2（StmP）是较小的相比，与Stm的增长。在StmP的存在下生长的晶体的形状比在Stm的存在下获得的那些更光滑和更球形。晶体尺寸的减小取决于蛋白质浓度的水平，表明Stm和StmP充当晶体生长的抑制剂。
The inner ear of the Danio rerio fish contains biominerals called otoliths that are composed of calcium carbonate crystals and a protein organic matrix. It has been previously suggested that Starmaker (Stm), which is an intrinsically disordered protein, acts as a component of otoliths that controls the size, shape, and polymorph of crystals. In this study, an in vitro calcium carbonate crystallization system was established to examine the role of Stm and extensively phosphorylated Stm (StmP) in the formation of crystals. SEM and X-ray diffraction analyses indicated that the dimensions of calcite crystals growing in the presence of Stm that had been phosphorylated by CK2 (StmP) were smaller in comparison with those growing with Stm. The shape of crystals growing in the presence of StmP were smoother and more spherical than those obtained in the presence of Stm. The decrease in crystal size, depending on the level of protein concentration, indicates that Stm and StmP act as inhibitors of crystal growth.