NEOPLASTIC DIFFERENTIATION - CHARACTERISTICS OF CELL LINES DERIVED FROM A MURINE TERATOCARCINOMA
NEOPLASTIC DIFFERENTIATION - CHARACTERISTICS OF CELL LINES DERIVED FROM A MURINE TERATOCARCINOMA
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DOI：
10.1002/jcp.1040840103
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
PIERCE, GB
中科院分区：
文献类型：
--
作者：
LEHMAN, JM;SPEERS, WC;PIERCE, GB
建立了小鼠畸胎癌细胞的体外单层培养。这些培养物含有胚胎癌、恶性干细胞及其分化的后代：壁卵黄囊、神经上皮细胞和间充质细胞。组织，如鳞状上皮，软骨，横纹肌，神经上皮，和腺体产生的胚胎癌，保持在长期培养的条件下。用胰酶频繁传代培养，可以建立胚胎癌细胞系，这些细胞系在体外维持超过18个月，并在特定培养条件下继续产生分化细胞。染色体上，这些胚胎癌细胞系具有39条染色体的干细胞系。已经建立了两个壁卵黄囊细胞系，它们产生基底膜，不致瘤，染色体是亚四倍体。该系统可以产生关于肿瘤分化及其在癌症治疗中的可能用途的信息。
Monolayer cultures of a mouse teratocarcinoma were established in vitro. These cultures contained embryonal carcinoma, the malignant stem cell, and its differentiated progeny: parietal yolk sac, neuroepithelial, and mesenchymal cells. Tissues such as squamous epithelium, cartilage, striated muscle, neuroepithelium, and glands were produced from embryonal carcinoma that was maintained under conditions of long term culture. Frequent subcultivation with pancreatin allowed the establishment of cell lines of embryonal carcinoma which have been maintained for more than 18 months in vitro and continue to produce differentiated cells under specific culture conditions. Chromosomally these lines of embryonal carcinoma have a stem line of 39 chromosomes. Two lines of parietal yolk sac cells have been established which produce basement membrane, are not tumorigenic, and chromosomally are hypotetraploid. This system may yield information concerning neoplastic differentiation and its possible use in therapy for cancer.